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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab219186-10μl |
10μl |
现货 ![]() |
| |
| Ab219186-50μl |
50μL |
现货 ![]() |
| |
| Ab219186-100μl |
100μL |
现货 ![]() |
| |
| Ab219186-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | beta Actin Mouse mAb (AF647) |
|---|---|
| 别名 | beta Actin 抗体 | Actin 抗体 |
| 英文别名 | A26C1A antibody | A26C1B antibody | ACTB antibody | ACTB_HUMAN antibody | Actin beta antibody | Actin cytoplasmic 1 antibody | Actin, cytoplasmic 1, N-terminally processed antibody | Actx antibody | b actin antibody | b-actin antibody | β actin antibody | |
| 规格或纯度 | ExactAb™, 已验证, 0.5 mg/mL |
| 生化机理 | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | beta actin |
| 种属反应性 | 人(Human),大鼠(Rat),小鼠(Mouse) |
| 预测反应种属 | 兔(Rabbit),牛(Cow),狗(Dog),猪(Pig),鸡(Chicken) |
| 免疫原 | Synthetic peptide in beta Actin |
| 阳性对照 | IF/ICC: Hela Flow Cytometry: Jurkat |
| 偶联 | AF647 |
| Excitation(nm) | Red Laser(633nm) |
| Emission(nm) | 668nm |
| Ex/Em(nm) | Ex:650nm;Em:668nm |
| 克隆类型 | 单克隆抗体 |
|---|---|
| Format | Whole IgG |
| 轻链亚型 | kappa |
| 纯化方法 | Protein A purified |
| 储存缓冲液 | Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300. |
| 防腐剂 | 0.05% Proclin300 |
| 浓度 | 0.5 mg/mL |
| 储存温度 | 避光,-20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at -20°C. Protect from light. Avoid freeze/thaw cycles. |
| 分子类型 | 抗体 |
beta Actin Mouse mAb (AF647) (Ab219186) - IF/ICC
IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (AF647) (Ab219186) at 1/1000 dilution in blocking buffer overnight at 4°C. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
beta Actin Mouse mAb (AF647) (Ab219186) - Flow Cytometry
Flow cytometry analysis of beta Actin expression by Jurkat cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with beta Actin Mouse mAb (AF647) (Ab219186) (Red) at 1/1000 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF647) (Ab190077).
| 应用名称 | 稀释比例 |
|---|---|
| IF/ICC |
1/100-1/1000
|
| Flow Cytometry |
1:100-1:2000
|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab219186 | |
| 分析证书 | Ab219186 | |
| 分析证书 | Ab219186 |