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beta Actin Mouse mAb (AF647)

    应用:
  • Flow Cytometry
  • IF/ICC
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
  • 亚型: Mouse IgG1
  • 克隆号: FG01/2D10
  • 偶联: AF647
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab219186-10μl
10μl 现货 Stock Image
Ab219186-50μl
50μL 现货 Stock Image
Ab219186-100μl
100μL 现货 Stock Image
Ab219186-1ml
1ml 期货 Stock Image

基本信息

产品名称 beta Actin Mouse mAb (AF647)
别名 beta Actin 抗体 | Actin 抗体
英文别名 A26C1A antibody | A26C1B antibody | ACTB antibody | ACTB_HUMAN antibody | Actin beta antibody | Actin cytoplasmic 1 antibody | Actin, cytoplasmic 1, N-terminally processed antibody | Actx antibody | b actin antibody | b-actin antibody | β actin antibody |
规格或纯度 ExactAb™, 已验证, 0.5 mg/mL
生化机理 Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
宿主种属 小鼠(Mouse)
特异性 beta actin
种属反应性 人(Human),大鼠(Rat),小鼠(Mouse)
预测反应种属 兔(Rabbit),牛(Cow),狗(Dog),猪(Pig),鸡(Chicken)
免疫原 Synthetic peptide in beta Actin
阳性对照 IF/ICC: Hela Flow Cytometry: Jurkat
偶联 AF647
Excitation(nm) Red Laser(633nm)
Emission(nm) 668nm
Ex/Em(nm) Ex:650nm;Em:668nm

产品属性

克隆类型 单克隆抗体
Format Whole IgG
轻链亚型 kappa
纯化方法 Protein A purified
储存缓冲液 Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂 0.05% Proclin300
浓度 0.5 mg/mL
储存温度 避光,-20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
分子类型 抗体

关联靶点(人)

ACTB Tbio 肌动蛋白,细胞质 1(Actin, cytoplasmic 1) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

beta Actin Mouse mAb (AF647) (Ab219186) - IF/ICC
IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (AF647) (Ab219186) at 1/1000 dilution in blocking buffer overnight at 4°C. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

beta Actin Mouse mAb (AF647) (Ab219186) - Flow Cytometry
Flow cytometry analysis of beta Actin expression by Jurkat cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with beta Actin Mouse mAb (AF647) (Ab219186) (Red) at 1/1000 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF647) (Ab190077).

应用

应用名称 稀释比例
IF/ICC

1/100-1/1000

Flow Cytometry

1:100-1:2000

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批号(Lot Number) 证书类型 货号
ZJ25F0622396 分析证书 Ab219186
ZJ25F0622395 分析证书 Ab219186
ZJ25F0622394 分析证书 Ab219186

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