alpha Tubulin Mouse mAb (Biotin)

COA

级别和纯度:

已验证

ExactAb™

0.5 mg/mL

应用:

Flow Cytometry
IF/ICC
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Mouse IgG2b
克隆号: AB01/5A12
偶联: Biotin

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
Ab219159-10μl	10μl	现货
Ab219159-50μl	50μL	现货
Ab219159-100μl	100μL	现货
Ab219159-1ml	1ml	现货

查看相关系列

点击查看靶蛋白信息: TUBA1B

Accession#:P68363 (10) Gene ID:10376 (10) K-ALPHA-1 (7) TUBA1B (10) 生物素 (246)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本信息

产品名称	alpha Tubulin Mouse mAb (Biotin)
别名	alpha Tubulin 抗体 \| Tubulin 抗体
英文别名	Alpha tubulin ubiquitous antibody \| Alpha-tubulin ubiquitous antibody \| K alpha 1 antibody \| TBA1B_HUMAN antibody \| TUBA1B antibody \| Tubulin alpha 1B antibody \| Tubulin alpha 1B chain antibody \| Tubulin alpha ubiquitous antibody \| Tubulin alpha ubiquitou
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
宿主种属	小鼠(Mouse)
特异性	alpha tubulin
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
免疫原	Recombinant human alpha tubulin
阳性对照	WB: HeLa, MCF-7, HepG2, NIH3T3 and C6 whole cell lysates Flow Cytometry: Hela cells IF/ICC: Hela cells
偶联	Biotin

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	避光,-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
分子类型	抗体

关联靶点（人）

TUBA1B Tchem 微管蛋白 α-1B 链(Tubulin alpha-1B chain) (0 活性数据)

点击查看靶蛋白信息： TUBA1B

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

IF/ICC: alpha Tubulin Mouse mAb (Biotin) (Ab219159)

alpha Tubulin Mouse mAb (Biotin) (Ab219159) – IF/ICC
IF analysis of alpha Tubulin (red) in Hela cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes and permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were then stained with alpha Tubulin Mouse mAb (Biotin) (Ab219159) at a dilution of 1/1000 in blocking buffer for 2 hour at RT and then incubated with Recombinant Streptavidin Protein (AF647) (rp181946) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Western Blot: alpha Tubulin Mouse mAb (Biotin) (Ab219159)

alpha Tubulin Mouse mAb (Biotin) (Ab219159) - Western Blot
All lanes: alpha Tubulin Mouse mAb (Biotin) (Ab219159) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: SA-HRP (np156148) at 1/10000 dilution

Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 120.0 s

Flow Cytometry: alpha Tubulin Mouse mAb (Biotin) (Ab219159)

alpha Tubulin Mouse mAb (Biotin) (Ab219159) - Flow Cytometry
Flow cytometry analysis of alpha Tubulin expression by Hela cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with alpha Tubulin Mouse mAb (Biotin) (Ab219159) (red) at 1/50 dilution for 30 minutes at 4°C in the dark. Then incubated with Streptavidin protein (FITC) (rp155876) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
IF/ICC	1/100-1/1000
WB	1/100-1/1000
Flow Cytometry	1/50

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到4个结果

批号(Lot Number)	证书类型	货号
ZJ25F0826373	分析证书	Ab219159
ZJ25F0826374	分析证书	Ab219159
ZJ25F0826375	分析证书	Ab219159
ZJ25F0826376	分析证书	Ab219159

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度