PCNA Mouse mAb (AF488)

COA

级别和纯度:

已验证

ExactAb™

0.5 mg/mL

应用:

Flow Cytometry
IF/ICC

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human), 大鼠(Rat), 小鼠(Mouse)
亚型: Mouse IgG1
克隆号: C04/3D7
偶联: AF488

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货号 (SKU)	包装规格	是否现货
Ab219142-10μl	10μl	现货
Ab219142-50μl	50μL	现货
Ab219142-100μl	100μL	现货
Ab219142-1ml	1ml	期货

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基本信息

产品名称	PCNA Mouse mAb (AF488)
别名	PCNA 抗体
英文别名	ATLD2 antibody \| cb16 antibody \| Cyclin antibody \| DNA polymerase delta auxiliary protein antibody \| etID36690.10 antibody \| fa28e03 antibody \| fb36g03 antibody \| HGCN8729 antibody \| MGC8367 antibody \| Mutagen-sensitive 209 protein antibody \| OTTHUMP00000
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
生化机理	Proliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication. Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix. Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA. PCNA protein expression is a well-accepted marker of proliferation.
宿主种属	小鼠(Mouse)
特异性	PCNA
种属反应性	人(Human),大鼠(Rat),小鼠(Mouse)
预测反应种属	兔(Rabbit),牛(Cow),狗(Dog),猪(Pig),鸡(Chicken)
免疫原	Full length recombinant human PCNA
阳性对照	IF/ICC: Hela cells Flow Cytometry: Hela cells
偶联	AF488
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	525nm
Ex/Em(nm)	Ex:490nm;Em:525nm

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	避光,-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
分子类型	抗体

关联靶点（人）

PCNA Tchem 增殖细胞核抗原(Proliferating cell nuclear antigen) (0 活性数据)

点击查看靶蛋白信息： PCNA

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

ICC/IF: PCNA Mouse mAb (AF488) (Ab219142)

PCNA Mouse mAb (AF488) (Ab219142) - ICC/IF
IF analysis of PCNA (Green) in MCF-7 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with PCNA Mouse mAb (AF488) (Ab219142) at a dilution of 1/1000 in blocking buffer overnight at 4°C. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: PCNA Mouse mAb (AF488) (Ab219142)

PCNA Mouse mAb (AF488) (Ab219142) - Flow Cytometry
Flow cytometry analysis of PCNA expression by Hela cells (Red) compared to an isotype control (Blue). Cells were fixed and permeabilized with 100% methanol and then stained with PCNA Mouse mAb (AF488) (Ab219142) (Red) at 1/150 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF488) (Ab190245).

应用

应用名称	稀释比例
IF/ICC	1:50-1:500
Flow Cytometry	1:50-1:500

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批号(Lot Number)	证书类型	货号
ZJ25F0825938	分析证书	Ab219142
ZJ25F0825939	分析证书	Ab219142
ZJ25F0825940	分析证书	Ab219142

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