NQO1 Mouse mAb

COA

级别和纯度:

≥95%(SDS-PAGE)
0.5 mg/mL

应用:

ELISA
Flow Cytometry
WB

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG2b
克隆号: AB01/1C6
偶联: Unconjugated

有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab215220-10μl	10μl	现货
Ab215220-50μl	50μL	现货
Ab215220-100μl	100μL	现货
Ab215220-1ml	1ml	期货

查看相关系列

点击查看靶蛋白信息: NQO1

Accession#:P15559 (6) Gene ID: 1728 (2) NQO1 (6)

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基本信息

产品名称	NQO1 Mouse mAb
别名	NAD (P)H：醌氧化还原酶1 \| NQO1抗体 \| 小鼠抗人NQO1抗体 \| NQO1小鼠单克隆抗体
英文别名	Azoreductase antibody \| Cytochrome b 5 reductase antibody \| DHQU antibody \| DIA 4 antibody \| DIA4 antibody \| Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody \| Diaphorase (NADH/NADPH) antibody \| Diaphorase 4 antibody \| Dioxin inducible 1 antibo
规格或纯度	无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, ≥95%(SDS-PAGE), 0.5 mg/mL
生化机理	Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species (By similarity). Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion. Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential. Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome.
宿主种属	小鼠(Mouse)
特异性	Human NQO1
种属反应性	人(Human)
免疫原	Recombinant full length protein corresponding human NQO1
阳性对照	WB: A549, HeLa, MCF-7 and HepG2 whole cell lysates Flow Cytometry: MCF-7 cells
偶联	Unconjugated

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
SDS-PAGE	150.0 KDa
纯化方法	Protein A purified
储存缓冲液	PBS, pH 7.4
浓度	0.5 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融.
分子类型	抗体

关联靶点（人）

NQO1 Tchem NAD(P)H脱氢酶[醌]1(NAD(P)H dehydrogenase [quinone] 1) (0 活性数据)

点击查看靶蛋白信息： NQO1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Western Blot: NQO1 Mouse mAb (Ab215220)

NQO1 Mouse mAb (Ab215220) - Western Blot
All lanes: NQO1 Mouse Antibody (Ab215220) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 31 kDa
Observed band size: 30 kDa
Exposure time: 30.0 s

Flow Cytometry: NQO1 Mouse mAb (Ab215220)

NQO1 Mouse mAb (Ab215220) - Flow Cytometry 
Overlay histogram showing MCF-7 cells stained with NQO1 Mouse mAb (Ab215220) (Red) at 1.0 μg/mL for 1 hour at 4°C. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. Mouse IgG2b-kappa, anti-E8L (Ab210600) was used as an isotype control (Blue).

应用

应用名称	稀释比例
WB	1/500-1/100000
Flow Cytometry	1/500

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0724114	分析证书	Ab215220
ZJ25F0724113	分析证书	Ab215220
ZJ25F0724112	分析证书	Ab215220

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