基本信息

产品名称	Recombinant MHC Class II Antibody (AF555)
别名	组织相容性复合体β（HLA-DMB）抗体 \| MHC Class II抗体
英文别名	D6S221E antibody \| DMA antibody \| DMB antibody \| DP beta 1 antibody \| DP beta 1 chain antibody \| DP(W4) beta chain antibody \| DPB 1 antibody \| DPB1 antibody \| DPB1_HUMAN antibody \| DRB antibody \| H2Ea antibody \| HLA class II histocompatibility antigen ant
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
生化机理	Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
宿主种属	大鼠(Rat)
特异性	MHC Class II
种属反应性	小鼠(Mouse)
免疫原	Activated C57BL/6 mouse spleen cells
阳性对照	Flow: C57BL/6 mouse splenocytes
偶联	AF555
Excitation(nm)	Green Laser(532nm)
Emission(nm)	568nm
Ex/Em(nm)	Ex:553nm;Em:568nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

HLA-DPB1 Tbio HLA II 类组织相容性抗原，DP β1 链(HLA class II histocompatibility antigen, DP beta 1 chain) (0 活性数据)

点击查看靶蛋白信息： HLA-DPB1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant MHC Class II Antibody (AF555) (Ab213993)

Recombinant MHC Class II Antibody (AF555) (Ab213993) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant MHC Class II Antibody (AF555) (Ab213993) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF555) (Ab211666).

Flow Cytometry: Recombinant MHC Class II Antibody (AF555) (Ab213993)

Recombinant MHC Class II Antibody (AF555) (Ab213993) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant MHC Class II Antibody (AF555) (Ab213993) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (AF555) (Ab211666). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	≤0.3μg/test For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.