Recombinant CD3 Antibody (APC)

COA

级别和纯度:

重组

已验证

ExactAb™

0.5 mg/mL

应用:

Flow Cytometry

宿主种属: 大鼠(Rat)
种属反应性: 小鼠(Mouse)
亚型: Rat IgG2b
克隆号: P49A24
偶联: APC

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货号 (SKU)	包装规格	是否现货
Ab213112-10μg	10μg	现货
Ab213112-25μg	25μg	现货
Ab213112-100μg	100μg	现货

查看相关系列

点击查看靶蛋白信息: CD3G

Accession#:P11942 (12) APC (180) Cd3g (8) Ctg-3 (10) Ctg3 (10) Gene ID:12502 (12) T3g (10)

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基本信息

产品名称	Recombinant CD3 Antibody (APC)
规格或纯度	ExactAb™, 已验证, 重组, 0.5 mg/mL
生化机理	Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition to this role of signal transduction in T-cell activation, CD3G plays an essential role in the dynamic regulation of TCR expression at the cell surface. Indeed, constitutive TCR cycling is dependent on the di-leucine-based (diL) receptor-sorting motif present in CD3G (PubMed:25920998).
宿主种属	大鼠(Rat)
特异性	CD3
种属反应性	小鼠(Mouse)
阳性对照	Flow Cytometry: C57BL/6 mouse splenocytes
偶联	APC
Excitation(nm)	Red Laser(633nm)
Emission(nm)	660nm
Ex/Em(nm)	Ex:650nm;Em:660nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
纯化方法	Protein G purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

CD3G Tclin T 细胞表面糖蛋白 CD3 γ 链(T-cell surface glycoprotein CD3 gamma chain) (0 活性数据)

点击查看靶蛋白信息： CD3G

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant CD3 Antibody (APC) (Ab213112)

Recombinant CD3 Antibody (APC) (Ab213112) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD3 Antibody (APC) (Ab213112) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (APC) (Ab180313).

Flow Cytometry: Recombinant CD3 Antibody (APC) (Ab213112)

Recombinant CD3 Antibody (APC) (Ab213112) - Flow Cytometry
Flow cytometry analysis of CD3 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD3 Antibody (APC) (Ab213112) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (APC) (Ab180313). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

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批号(Lot Number)	证书类型	货号
ZJ25F1028452	分析证书	Ab213112
ZJ25F1028451	分析证书	Ab213112
ZJ25F1028450	分析证书	Ab213112

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