c-Kit/CD117 Rat mAb (AF488)

COA

级别和纯度:

已验证

ExactAb™

0.5 mg/mL

应用:

Flow Cytometry

宿主种属: 大鼠(Rat)
种属反应性: 小鼠(Mouse)
亚型: Rat IgG2b
克隆号: ACK2
偶联: AF488

有货

库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab209965-10μg	10μg	现货
Ab209965-25μg	25μg	现货
Ab209965-100μg	100μg	现货

查看相关系列

点击查看靶蛋白信息: KIT

Accession#: P05532 (20) AF488 (107) Bs (14) CD117 (21) Fdc (13) Gene ID: 16590 (20) Gsfsco1 (14) Gsfsco5 (14) Gsfsow3 (14) SCO1 (14) SCO5 (14) SOW3 (14) Ssm (14) Tr-kit (14) W (12)

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基本信息

产品名称	c-Kit/CD117 Rat mAb (AF488)
别名	干细胞因子受体(SCFR)抗体 \| c-Kit/CD117抗体
英文别名	W \| Bs \| Fdc \| Ssm \| SCO1 \| SCO5 \| SOW3 \| c-KIT \| Tr-kit \| Gsfsco1 \| Gsfsco5 \| Gsfsow3 \| CD117 antigen \| CD117 \| ckit \| EC 2.7.10 \| EC 2.7.10.1 \| KIT \| PBT \| piebald trait \| Proto-oncogene c-Kit \| proto-oncogene tyrosine-protein kinase Kit \| SCF R \| SCFR
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
生化机理	Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
宿主种属	大鼠(Rat)
特异性	c-Kit/CD117
种属反应性	小鼠(Mouse)
免疫原	IL-3-dependent mast cells
阳性对照	Flow: C57BL/6 mouse bone marrow cells
偶联	AF488
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	525nm
Ex/Em(nm)	Ex:490nm;Em:525nm

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein G purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

KIT Tclin 肥大细胞/干细胞生长因子受体试剂盒(Mast/stem cell growth factor receptor Kit) (0 活性数据)

点击查看靶蛋白信息： KIT

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: c-Kit/CD117 Rat mAb (AF488) (Ab209965)

c-Kit/CD117 Rat mAb (AF488) (Ab209965) - Flow Cytometry
Two-color flow cytometric analysis of c-Kit/CD117 expression on C57BL/6 mouse bone marrow cells. C57BL/6 mouse bone marrow cells preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD45R Rat mAb (AF647) (Ab191663) and either Rat IgG (AF488) (Ab190240; Left panel) or c-Kit/CD117 Rat mAb (AF488) (Ab209965; Right panel) at 0.5μg/test.

应用

应用名称	稀释比例
Flow Cytometry	≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0825155	分析证书	Ab209965
ZJ25F0825154	分析证书	Ab209965
ZJ25F0825153	分析证书	Ab209965

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