SIRP alpha/CD172a Rat mAb (AF488)

COA

级别和纯度:

已验证

ExactAb™

0.5 mg/mL

应用:

Flow Cytometry

宿主种属: 大鼠(Rat)
种属反应性: 小鼠(Mouse)
亚型: Rat IgG1
克隆号: P84
偶联: AF488

有货

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab191790-10μg	10μg	现货
Ab191790-25μg	25μg	现货
Ab191790-100μg	100μg	现货

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点击查看靶蛋白信息: SIRPA

Accession#: P97797 (10) AF488 (107) Bit (9) CD172a (11) Gene ID: 19261 (10) Idd13.2 (9) P84 (9) Ptpns1 (9) SHP-1 (10) SHPS-1 (9) SIRP (9)

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基本信息

产品名称	SIRP alpha/CD172a Rat mAb (AF488)
别名	信号调节蛋白α(SIRPa)抗体 \| SIRP alpha/CD172a抗体
英文别名	Bit \| SIRP \| SHP-1 \| Ptpns1 \| Idd13.2 \| BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs \| Brain Ig-like molecule with tyrosine-based activation motifs \| CD172 antigen-like family member A \| CD172a antigen \| CD172a \| Inhibitory
规格或纯度	ExactAb™, 已验证, 0.5 mg/mL
生化机理	Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
宿主种属	大鼠(Rat)
特异性	SIRP alpha/CD172a
种属反应性	小鼠(Mouse)
免疫原	Mouse brain membrane protein
阳性对照	Flow: C57BL/6 mouse bone marrow cells.
偶联	AF488
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	525nm
Ex/Em(nm)	Ex:490nm;Em:525nm

产品属性

克隆类型	单克隆抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5 mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

SIRPA Tbio 酪氨酸蛋白磷酸酶非受体型底物 1(Tyrosine-protein phosphatase non-receptor type substrate 1) (0 活性数据)

点击查看靶蛋白信息： SIRPA

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: SIRP alpha/CD172a Rat mAb (AF488) (Ab191790)

SIRP alpha/CD172a Rat mAb (AF488) (Ab191790) - Flow Cytometry
Flow cytometry analysis of SIRP alpha/CD172a expression by C57BL/6 mouse bone marrow cells (right panel) compared to an isotype control (left panel). Cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with SIRP alpha/CD172a Rat mAb (AF488) (Ab191790) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF488) (Ab190240).

Flow Cytometry: SIRP alpha/CD172a Rat mAb (AF488) (Ab191790)

SIRP alpha/CD172a Rat mAb (AF488) (Ab191790) - Flow Cytometry
Flow cytometry analysis of SIRP alpha/CD172a expression by C57BL/6 mouse bone marrow cells (red) compared to an isotype control (blue). Cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with SIRP alpha/CD172a Rat mAb (AF488) (Ab191790) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF488) (Ab190240). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0825216	分析证书	Ab191790
ZJ25F0825215	分析证书	Ab191790
ZJ25F0825214	分析证书	Ab191790

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