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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab191775-10T |
10T |
现货 ![]() |
| |
| Ab191775-25T |
25T |
现货 ![]() |
| |
| Ab191775-100T |
100T |
现货 ![]() |
|
| 产品名称 | IFN-γ Mouse mAb (FITC) |
|---|---|
| 别名 | 干扰素γ(IFNg)抗体 | Interferon gamma抗体 |
| 英文别名 | IMD69 | IFG | IFI | IFNG | IFNgamma | IFN-gamma | Immune interferon | interferon gamma | interferon, gamma | IF 1 antibody | IFG antibody | IFI antibody | ifn g antibody | IFN gamma antibody | IFN immune antibody | IFN, immune antibody | IFN-gamma antibod |
| 规格或纯度 | ExactAb™, 已验证, 5 μL/test |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | Interferon gamma |
| 种属反应性 | 人(Human) |
| 免疫原 | Recombinant protein of human IFNγ |
| 阳性对照 | Flow Cytometry: Human peripheral blood lymphocytes |
| 偶联 | FITC |
| Excitation(nm) | Blue Laser(488nm) |
| Emission(nm) | 517nm |
| Ex/Em(nm) | Ex:498nm;Em:517nm |
| 克隆类型 | 单克隆抗体 |
|---|---|
| Format | Whole IgG |
| 纯化方法 | Protein G purified |
| 储存缓冲液 | Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300. |
| 防腐剂 | 0.05% Proclin300 |
| 浓度 | 5 μL/test |
| 储存温度 | 2-8°C储存,避光,禁止冷冻 |
| 运输条件 | 冰袋运输 |
| 稳定性与储存 | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| 分子类型 | 抗体 |
IFN-γ Mouse mAb (FITC) (Ab191775) - Flow Cytometry
Expression of IFN-γ by stimulated human peripheral blood lymphocyte cells (PBMC). Human PBMC were stimulated for 5 hours with PMA (50 ng/ml) plus ionomycin (1 μg/mL) and brefeldin A (3 μg/mL) for 2 hours. The PBMC were stained with CD3 Mouse mAb (AF647) (Ab189663), fixed with 2% PFA for 30 minutes, permeabilized with 0.5% triton X-100 for 30 minutes, then subsequently stained with 0.1 µg of IFN-γ Mouse mAb (FITC) (Ab191775; Right panel). Unstimulated Human PBMC were used as control (Left panel).
IFN-γ Mouse mAb (FITC) (Ab191775) - Flow Cytometry
Expression of IFN-γ by stimulated human peripheral blood lymphocyte cells (PBMC). Human PBMC were stimulated for 5 hours with PMA (50 ng/ml) plus ionomycin (1 μg/mL) and brefeldin A (3 μg/mL) for 2 hours. The PBMC were stained with CD3 Mouse mAb (AF647) (Ab189663), fixed with 2% PFA for 30 minutes, permeabilized with 0.5% triton X-100 for 30 minutes, then subsequently stained with 0.1 µg of IFN-γ Mouse mAb (FITC) (Ab191775; Right panel). Left panel - Mouse IgG (FITC) (Ab180307).
| 应用名称 | 稀释比例 |
|---|---|
| Flow Cytometry | 5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab191775 | |
| 分析证书 | Ab191775 | |
| 分析证书 | Ab191775 |