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IFN-γ Mouse mAb (FITC)

    应用:
  • Flow Cytometry
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG1
  • 克隆号: B27
  • 偶联: FITC
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab191775-10T
10T 现货 Stock Image
Ab191775-25T
25T 现货 Stock Image
Ab191775-100T
100T 现货 Stock Image

基本信息

产品名称 IFN-γ Mouse mAb (FITC)
别名 干扰素γ(IFNg)抗体 | Interferon gamma抗体
英文别名 IMD69 | IFG | IFI | IFNG | IFNgamma | IFN-gamma | Immune interferon | interferon gamma | interferon, gamma | IF 1 antibody | IFG antibody | IFI antibody | ifn g antibody | IFN gamma antibody | IFN immune antibody | IFN, immune antibody | IFN-gamma antibod
规格或纯度 ExactAb™, 已验证, 5 μL/test
宿主种属 小鼠(Mouse)
特异性 Interferon gamma
种属反应性 人(Human)
免疫原 Recombinant protein of human IFNγ
阳性对照 Flow Cytometry: Human peripheral blood lymphocytes
偶联 FITC
Excitation(nm) Blue Laser(488nm)
Emission(nm) 517nm
Ex/Em(nm) Ex:498nm;Em:517nm

产品属性

克隆类型 单克隆抗体
Format Whole IgG
纯化方法 Protein G purified
储存缓冲液 Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂 0.05% Proclin300
浓度 5 μL/test
储存温度 2-8°C储存,避光,禁止冷冻
运输条件 冰袋运输
稳定性与储存 The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型 抗体

关联靶点(人)

IFNG Tclin 干扰素γ(Interferon gamma) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

IFN-γ Mouse mAb (FITC) (Ab191775) - Flow Cytometry
Expression of IFN-γ by stimulated human peripheral blood lymphocyte cells (PBMC). Human PBMC were stimulated for 5 hours with PMA (50 ng/ml) plus ionomycin (1 μg/mL) and brefeldin A  (3 μg/mL) for 2 hours. The PBMC were stained with CD3 Mouse mAb (AF647) (Ab189663), fixed with 2% PFA for 30 minutes, permeabilized with 0.5%  triton X-100 for 30 minutes, then subsequently stained with 0.1 µg of IFN-γ Mouse mAb (FITC) (Ab191775; Right panel). Unstimulated Human PBMC were used as control (Left panel).

IFN-γ Mouse mAb (FITC) (Ab191775) - Flow Cytometry
Expression of IFN-γ by stimulated human peripheral blood lymphocyte cells (PBMC). Human PBMC were stimulated for 5 hours with PMA (50 ng/ml) plus ionomycin (1 μg/mL) and brefeldin A  (3 μg/mL) for 2 hours. The PBMC were stained with CD3 Mouse mAb (AF647) (Ab189663), fixed with 2% PFA for 30 minutes, permeabilized with 0.5%  triton X-100 for 30 minutes, then subsequently stained with 0.1 µg of IFN-γ Mouse mAb (FITC) (Ab191775; Right panel). Left panel - Mouse IgG (FITC) (Ab180307).

应用

应用名称 稀释比例
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ25F0825219 分析证书 Ab191775
ZJ25F0825218 分析证书 Ab191775
ZJ25F0825217 分析证书 Ab191775

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