基本信息

产品名称	Demupitamab (anti-EGFR)
英文别名	Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody \| Cell growth inhibiting protein 40 antibody \| Cell proliferation inducing protein 61 antibody \| EGF R antibody \| EGFR antibody \| EGFR_HUMAN antibody \| Epidermal growth factor recepto
规格或纯度	无载体, 重组, ExactAb™, 低内毒素, 无叠氮钠, 已验证, 无动物源, ≥95%(SDS-PAGE&SEC-HPLC), 见COA
生化机理	Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.
宿主种属	人(Human)
特异性	EGFR
种属反应性	人(Human)
偶联	Unconjugated

产品属性

克隆类型	重组抗体
Format	Whole IgG
亚型	Human IgG1
轻链亚型	kappa
SDS-PAGE	144.8 kDa
纯化方法	Protein A purified
储存缓冲液	Supplied as a 0.22μm filtered solution in 100mM Pro-Ac, 20mM Arg, pH 5.0
防腐剂	No
浓度	见COA
储存温度	-80℃储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -80℃ 下保存 24 个月。收货后建议分装。避免冷冻/解冻循环。
CAS编号和信息	2249926-74-5
分子类型	抗体

关联靶点（人）

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EGFR Tclin MER intracellular domain/EGFR extracellular domain chimera (2 活性数据)

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EGFR Tclin Protein cereblon/Epidermal growth factor receptor (191 活性数据)

点击查看靶蛋白信息： EGFR

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EGFR Tclin EGFR/PPP1CA (1 活性数据)

点击查看靶蛋白信息： EGFR

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EGFR Tclin VHL/EGFR (323 活性数据)

点击查看靶蛋白信息： EGFR

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

图片

Flow Cytometry: Demupitamab (anti-EGFR) (Ab190141)

Demupitamab (anti-EGFR) (Ab190141) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling EGFR (red) with Demupitamab (anti-EGFR) (Ab190141) at 1.0 μg/mL for 1 hour at 4°C. Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

ELISA: Demupitamab (anti-EGFR) (Ab190141)

Demupitamab (anti-EGFR) (Ab190141) - ELISA
Immobilized Recombinant Human EGFR protein (rp176273) at 1.0 μg/mL can bind Demupitamab (anti-EGFR) (Ab190141) with the EC50 of 36.15 ng/mL.