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BrdU Mouse mAb

    应用:
  • Flow Cytometry
  • IF/ICC
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 不依赖种属(Species independent)
  • 亚型: Mouse IgG1
  • 克隆号: AB04/2F8
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab179917-10μl
10μl 现货 Stock Image
Ab179917-50μl
50μL 现货 Stock Image
Ab179917-100μl
100μL 现货 Stock Image
Ab179917-1ml
1ml 期货 Stock Image
查看相关系列
BrdU (6) 流式细胞术 (1163)

基本信息

产品名称 BrdU Mouse mAb
别名 BrdU 小鼠单克隆抗体 | BrdU 小鼠单抗
英文别名 Bromodeoxyuridine antibody | BrdU antibody | Anti-BrdU antibody | 5-bromo-2-deoxyuridine antibody | BrdU Monoclonal Antibody | Anti-BrdU/5-BrdU Antibody | Anti BrdU
规格或纯度 ExactAb™, Validated, Carrier Free, Azide Free, High performance, 0.5 mg/mL
宿主种属 小鼠(Mouse)
种属反应性 不依赖种属(Species independent)
免疫原 Bromodeoxyuridine
阳性对照 Flow: Jurkat cells; ICC/IF: HeLa cells
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 AB04/2F8
Format Whole IgG
亚型 IgG1
轻链亚型 lambda
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 PBS, pH 7.2
浓度 0.5 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 在 +4°C 下短期储存(1-2 周)。-20°C或-80°C储存。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

图片

BrdU Mouse mAb (Ab179917) - Flow Cytometry
Dot plot showing BrdU-treated (A) and DMSO-treated (B) Jurkat cells stained with Ab179917. Cells were incubated with 10 µM BrdU for 1 hour prior to being harvested. And then fixed in 100% methanol for 20 minutes at -20℃. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at room temperature.
Cells were stained with 0.32 μg/mL BrdU Mouse mAb (Ab179917) for 1 hour at room temperature. Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as a secondary antibody at a 1/1000 dilution. Propidium Iodide was used to stain total DNA.

BrdU Mouse mAb (Ab179917) - ICC/IF
IF analysis of HeLa cells labeling BrdU with BrdU Mouse mAb (Ab179917) at 0.5 μg/mL. The cells were fixed with 100% methanol for 5 minutes and then subjected to acid hydrolysis using 2M HCl for 30 minutes at room temperature to denature the DNA. They were then blocked with 2% BSA in PBS for 1 hour and incubated overnight at 4℃ with BrdU Mouse mAb (Ab179917) at 0.5 μg/mL.
beta Tubulin Mouse mAb (Ab155823) (FITC) (shown in green) counterstained with the cells after Goat Anti-Mouse Cy5 (Ab156257) (shown in red) incubation. Nuclear DNA was labelled with DAPI (shown in blue).
Images were taken on the confocal laser scanning microscope.

应用

应用名称 稀释比例
IF/ICC

0.5 μg/mL

Flow Cytometry

0.16 μg/mL - 5.0 μg/mL

质检证书(CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ23F1202280 分析证书 Ab179917
ZJ23F1202281 分析证书 Ab179917
ZJ23F1202282 分析证书 Ab179917

技术文档和文章

采用碘化丙啶DNA染色法进行流式细胞术细胞周期分析
流式实验样品制备实验指南
Experimental Guide of Sample Preparation for Flow Cytometry
Experimental Guide of Sample Preparation for Flow Cytometry
BrdU染色方案
BrdU staining protocol
BrdU staining protocol
BrdU staining protocol
免疫荧光染色实验步骤(甲醇通透法)
Immunofluorescence Staining Protocol (Methanol permeabilization)
Immunofluorescence Staining Protocol (Methanol permeabilization)
利用甲醇通透法进行流式细胞术
Flow Cytometry Using Methanol Permeabilization
Flow Cytometry Using Methanol Permeabilization

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