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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab179917-10μl |
10μl |
现货 ![]() |
| |
| Ab179917-50μl |
50μL |
现货 ![]() |
| |
| Ab179917-100μl |
100μL |
现货 ![]() |
| |
| Ab179917-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | BrdU Mouse mAb |
|---|---|
| 别名 | BrdU 小鼠单克隆抗体 | BrdU 小鼠单抗 |
| 英文别名 | Bromodeoxyuridine antibody | BrdU antibody | Anti-BrdU antibody | 5-bromo-2-deoxyuridine antibody | BrdU Monoclonal Antibody | Anti-BrdU/5-BrdU Antibody | Anti BrdU |
| 规格或纯度 | ExactAb™, Validated, Carrier Free, Azide Free, High performance, 0.5 mg/mL |
| 宿主种属 | 小鼠(Mouse) |
| 种属反应性 | 不依赖种属(Species independent) |
| 免疫原 | Bromodeoxyuridine |
| 阳性对照 | Flow: Jurkat cells; ICC/IF: HeLa cells |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| 克隆号 | AB04/2F8 |
| Format | Whole IgG |
| 亚型 | IgG1 |
| 轻链亚型 | lambda |
| 纯化方法 | Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | PBS, pH 7.2 |
| 浓度 | 0.5 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | 在 +4°C 下短期储存(1-2 周)。-20°C或-80°C储存。收货后建议分装。避免冷冻/解冻循环。 |
| 分子类型 | 抗体 |
BrdU Mouse mAb (Ab179917) - Flow Cytometry
Dot plot showing BrdU-treated (A) and DMSO-treated (B) Jurkat cells stained with Ab179917. Cells were incubated with 10 µM BrdU for 1 hour prior to being harvested. And then fixed in 100% methanol for 20 minutes at -20℃. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at room temperature.
Cells were stained with 0.32 μg/mL BrdU Mouse mAb (Ab179917) for 1 hour at room temperature. Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as a secondary antibody at a 1/1000 dilution. Propidium Iodide was used to stain total DNA.
BrdU Mouse mAb (Ab179917) - ICC/IF
IF analysis of HeLa cells labeling BrdU with BrdU Mouse mAb (Ab179917) at 0.5 μg/mL. The cells were fixed with 100% methanol for 5 minutes and then subjected to acid hydrolysis using 2M HCl for 30 minutes at room temperature to denature the DNA. They were then blocked with 2% BSA in PBS for 1 hour and incubated overnight at 4℃ with BrdU Mouse mAb (Ab179917) at 0.5 μg/mL.
beta Tubulin Mouse mAb (Ab155823) (FITC) (shown in green) counterstained with the cells after Goat Anti-Mouse Cy5 (Ab156257) (shown in red) incubation. Nuclear DNA was labelled with DAPI (shown in blue).
Images were taken on the confocal laser scanning microscope.
| 应用名称 | 稀释比例 |
|---|---|
| IF/ICC |
0.5 μg/mL
|
| Flow Cytometry | 0.16 μg/mL - 5.0 μg/mL |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab179917 | |
| 分析证书 | Ab179917 | |
| 分析证书 | Ab179917 |