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BrdU Mouse mAb

COA COA
    应用:
  • Flow Cytometry
  • IF/ICC
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 不依赖种属(Species independent)
  • 亚型: Mouse IgG1
  • 克隆号: AB06/4C1
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab176205-10μl
 10μl 现货 Stock Image
Ab176205-50μl
 50μL 现货 Stock Image
Ab176205-100μl
 100μL 现货 Stock Image
Ab176205-1ml
 1ml 期货 Stock Image
大包装询价 收藏夹 比较  SDS下载  DATASHEET  质量标准
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BrdU (6) 流式细胞术 (1163)
main product photo

基本信息

产品名称 BrdU Mouse mAb
别名 BrdU 小鼠单克隆抗体 | BrdU 小鼠单抗
英文别名 Bromodeoxyuridine antibody | BrdU antibody | Anti-BrdU antibody | 5-bromo-2-deoxyuridine antibody | BrdU Monoclonal Antibody | Anti-BrdU/5-BrdU Antibody | Anti BrdU
规格或纯度 无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
宿主种属 小鼠(Mouse)
种属反应性 不依赖种属(Species independent)
免疫原 Bromodeoxyuridine
阳性对照 Flow: Jurkat cells; ICC/IF: HeLa cells
偶联 Unconjugated

产品属性

克隆类型 单克隆抗体
克隆号 AB06/4C1
Format Whole IgG
亚型 IgG1
轻链亚型 kappa
SDS-PAGE 150 kDa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 PBS, pH 7.2
防腐剂 No
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 在 +4°C 下短期储存（1-2 周）。-20°C或-80°C储存。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

图片

BrdU Mouse mAb (Ab176205) - Flow Cytometry
Dot plot showing BrdU-treated (A) and DMSO-treated (B) Jurkat cells stained with Ab176205. Cells were incubated with 10 µM BrdU for 1 hour prior to being harvested. And then fixed in 100% methanol for 20 minutes at -20℃. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at room temperature.


Cells were stained with 0.1 μg/mL BrdU Mouse mAb (Ab176205) for 1 hour at room temperature. Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as a secondary antibody at a 1/1000 dilution. Propidium Iodide was used to stain total DNA.

BrdU Mouse mAb (Ab176205) - Flow Cytometry
Flow cytometry analysis of BrdU (red line) in Jurkat cells. The cells were treated with 10μM BrdU for 1 hour. And then fixed in 100% methanol for 20 minutes at -20℃. An acid denaturation method was used to prepare the cells before labeling with BrdU Mouse mAb (Ab176205) to detect the incorporated BrdU. Cells were then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at room temperature in the dark. Unlabeled sample (black line) was used as a negative control.

BrdU Mouse mAb (Ab176205) - ICC/IF
IF analysis of HeLa cells labeling BrdU with BrdU Mouse mAb (Ab176205) at 0.5 μg/mL. The cells were fixed with 100% methanol for 5 minutes and then subjected to acid hydrolysis using 2M HCl for 30 minutes at room temperature to denature the DNA. They were then blocked with 2% BSA in PBS for 1 hour and incubated overnight at 4℃ with BrdU Mouse mAb (Ab176205) at 0.5 μg/mL.

 
beta Tubulin Mouse mAb (Ab155823) (FITC) (shown in green) counterstained with the cells after Goat Anti-Mouse Cy5 (Ab156257) (shown in red) incubation. Nuclear DNA was labelled with DAPI (shown in blue).

 
Images were taken on the confocal laser scanning microscope.

BrdU Mouse mAb (Ab176205) - ICC/IF
IF analysis of HeLa cells labeling BrdU with BrdU Mouse mAb (Ab176205) at 0.5 μg/mL. The cells were fixed with 100% methanol for 5 minutes and then subjected to acid hydrolysis using 2M HCl for 30 minutes at room temperature to denature the DNA. They were then blocked with 2% BSA in PBS for 1 hour and incubated overnight at 4℃ with BrdU Mouse mAb (Ab176205) at 0.5 μg/mL.

 
beta Tubulin Mouse mAb (Ab155823) (FITC) (shown in green) counterstained with the cells after Goat Anti-Mouse Cy5 (Ab156257) (shown in red) incubation. Nuclear DNA was labelled with DAPI (shown in blue).

 
Images were taken on the confocal laser scanning microscope.

应用

应用名称 稀释比例
IF/ICC

0.5 μg/mL

Flow Cytometry

0.01 μg/mL- 2.0 μg/mL

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到6个结果

批号(Lot Number) 证书类型 货号
ZJ23F0901023 分析证书 Ab176205
ZJ23F0901024 分析证书 Ab176205
ZJ24F0102485 分析证书 Ab176205
ZJ24F0102484 分析证书 Ab176205
ZJ24F0102483 分析证书 Ab176205
ZJ24F0102474 分析证书 Ab176205

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