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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab121716-10μg |
10μg |
现货 ![]() |
| |
| Ab121716-50μg |
50μg |
现货 ![]() |
| |
| Ab121716-100μg |
100μg |
现货 ![]() |
| |
| Ab121716-1mg |
1mg |
期货 ![]() |
|
| 产品名称 | Phosphotyrosine Mouse mAb |
|---|---|
| 别名 | 磷酸化酪氨酸抗体 | Phosphotyrosine抗体 | 磷酸酪氨酸小鼠单克隆抗体 | 磷酸酪氨酸小鼠单抗 |
| 英文别名 | 4 hydroxyphenylalanine | PhosphoTyrosine | pTyrosine | pY20 | Tyrosine | p-Tyr | 2 amino 3(4 hydroxyphenyl) propanoic acid |
| 规格或纯度 | 无载体, ExactAb™, 无叠氮钠, 已验证, ≥95%(SDS-PAGE), 见COA |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | Phosphotyrosine |
| 种属反应性 | 不依赖种属(Species independent) |
| 免疫原 | Phosphotyramine-KLH |
| 偶联 | Unconjugated |
| 克隆类型 | 单克隆抗体 |
|---|---|
| 克隆号 | 4G10 |
| Format | Whole IgG |
| 亚型 | Mouse IgG2b |
| 轻链亚型 | kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein G purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, pH 7.0 |
| 浓度 | 见COA |
| 储存温度 | 2-8°C储存,禁止冷冻 |
| 运输条件 | 冰袋运输 |
| 稳定性与储存 | 4°C 下运输。4°C 长期保存(12 个月)。请勿冷冻。收货后建议分装。 |
| 分子类型 | 抗体 |
Phosphotyrosine Mouse mAb (Ab121716) - Flow Cytometry
Flow Cytometry analysis of unstimulated (left) or 1 mM sodium vanadate (S105283) stimulated (right) NIH3T3 mouse embryonic fibroblast cells labelling Phosphotyrosine Mouse mAb (red) with Phosphotyrosine Mouse mAb (Ab121716) at 0.2 μg/mL for 1 hour at 4°C, and detected with Goat anti-Mouse IgG (PE) (Ab179006). Blue - Mouse IgG Isotype Control Antibody (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
Phosphotyrosine Mouse mAb (Ab121716) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells was cultured in serum-free media overnight and treated with 5 mM H2O2 for 15 minutes labelling Phosphotyrosine Mouse mAb (red) with Phosphotyrosine Mouse mAb (Ab121716) conjugated with biotin at 0.2 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
| 应用名称 | 稀释比例 |
|---|---|
| WB | Use at an assay dependent concentration. |
| Flow Cytometry | Use at an assay dependent concentration. |
| ELISA | Use at an assay dependent concentration. |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab121716 | |
| 分析证书 | Ab121716 | |
| 分析证书 | Ab121716 |