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Recombinant GST tag Antibody

COA COA
    应用:
  • Flow Cytometry
  • IF/ICC
  • IP
  • WB
  • 宿主种属: 兔(Rabbit)
  • 种属反应性: 不依赖种属(Species independent)
  • 亚型: Rabbit IgG
  • 偶联: Unconjugated
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab106638-10μl
 10μl 现货 Stock Image
Ab106638-50μl
 50μL 现货 Stock Image
Ab106638-100μl
 100μL 现货 Stock Image
Ab106638-1ml
 1ml 期货 Stock Image
大包装询价 收藏夹 比较  SDS下载  DATASHEET  质量标准
查看相关系列
Accession#:P08515 (3) Glutathione S Transferase (1) GST Epitope Tag (1) GST tag (2) Tag (18) 流式细胞术 (1163)
main product photo

基本信息

产品名称 Recombinant GST tag Antibody
别名 GST tag抗体 | GST tag重组单克隆抗体 | GST tag标签抗体
英文别名 Glutathione S Transferase antibody | Glutathione S-transferase class-mu 26 kDa isozyme antibody | GST antibody | Sj26 antigen antibody | SjGST antibody | Glutathione STransferase | Glutathione S-Transferase | GST tag antibody | Anti- GST tag antibody
规格或纯度 重组, ExactAb™, 已验证, 高性能, 见COA
宿主种属 兔(Rabbit)
特异性 GST
种属反应性 不依赖种属(Species independent)
免疫原 Recombinant full length protein within Schistosoma japonicum GST (AA 1-218).
阳性对照 WB: GST transfected HEK293. IF/ICC: 293 cells transfected with N-terminal and C-terminal GST tagged gene. Flow: 293 cells transfected with C-terminal and N-terminal GST tagged gene. IP: 293 whole cell lysates transfected with C-terminal and N-terminal GST
偶联 Unconjugated

产品属性

克隆类型 重组抗体
克隆号 RR697
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Protein A purified
物理外观 Liquid
储存缓冲液 59% 10mM PBS, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.3
防腐剂 0.01% Sodium azide
浓度 见COA
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 4°C 短期储存（1-2 周）。-20°C下长期保存（24个月）。收货后建议分装。避免冷冻/解冻循环。
分子类型 抗体

图片

Recombinant GST tag Antibody (Ab106638) - Western Blot
All lanes: Recombinant GST tag Antibody (Ab106638) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 116 kDa
Observed band size: 53, 60, 112 kDa
Exposure time: 117.5 s

Recombinant GST tag Antibody (Ab106638) - IP
GST tag was immunoprecipitated from 0.2 mg of 293 whole cell lysates transfected with N-terminal GST tagged gene with Recombinant GST tag Antibody (Ab106638) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant GST tag Antibody (Ab106638) IP in 293 whole cell lysates transfected with N-terminal GST tagged gene
Lane 2: PBS instead of Recombinant GST tag Antibody (Ab106638) in 293 whole cell lysates transfected with N-terminal GST tagged gene
Lane 3: 293 whole cell lysates transfected with N-terminal GST tagged gene, 2 μg (input)
Exposure: 10.0 s

Recombinant GST tag Antibody (Ab106638) - IP
GST tag was immunoprecipitated from 0.2 mg of 293 whole cell lysates transfected with C-terminal GST tagged gene with Recombinant GST tag Antibody (Ab106638) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500
Lane 1: Recombinant GST tag Antibody (Ab106638) IP in 293 whole cell lysates transfected with C-terminal GST tagged gene
Lane 2: PBS instead of Recombinant GST tag Antibody (Ab106638) in 293 whole cell lysates transfected with C-terminal GST tagged gene
Lane 3: 293 whole cell lysate transfected with C-terminal GST tagged gene, 2 μg (input)
Exposure: 10.0 s

Recombinant GST tag Antibody (Ab106638) - IF/ICC
Recombinant GST tag Antibody (Ab106638) staining GST tag in 293 cells transfected with C-terminal GST tagged gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant GST tag Antibody (Ab106638) (1/40000) at 4°C. An AF594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AF594-conjugated Goat Anti-Rabbit IgG (1/500).

Recombinant GST tag Antibody (Ab106638) - IF/ICC
Recombinant GST tag Antibody (Ab106638) staining GST tag in 293 cells transfected with N-terminal GST tagged gene by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant GST tag Antibody (Ab106638) (1/40000) at 4°C. An AF594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An AF594-conjugated Goat Anti-Rabbit IgG (1/500).

Recombinant GST tag Antibody (Ab106638) - Flow CytoMetry
Overlay histogram showing 293 cells transfected with C-terminal (Red) and N-terminal (Blue) GST tagged gene  stained with Recombinant GST tag Antibody (Ab106638). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (Recombinant GST tag Antibody (Ab106638), 1/2000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit AF488 (IgG H+L)  at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

应用

应用名称 稀释比例
WB

1/2000 - 1/5000

Flow Cytometry

1/800 - 1/2000
IP

1/50

IF/ICC

1/10000 - 1/40000

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到6个结果

批号(Lot Number) 证书类型 货号
ZJ25F0420425 分析证书 Ab106638
ZJ25F0420424 分析证书 Ab106638
ZJ25F0420423 分析证书 Ab106638
ZJ24F0506208 分析证书 Ab106638
ZJ24F0506207 分析证书 Ab106638
ZJ24F0506206 分析证书 Ab106638

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