Grouped product items

SKU

Size

Availability

Price

Qty

Ab131268-10μg

10μg

$29.90

Ab131268-50μg

50μg

$69.90

Ab131268-100μg

100μg

$99.90

Ab131268-1mg

1mg

Available within 8-12 weeks(?)

$219.90

Basic Description

Product Name	TIM3/CD366 Rat mAb - Primary antibody, specific to TIM 3/CD366, Rat IgG2a
Synonyms	Tim3 \| TIM-3 \| Timd3 \| CD366 \| FLJ14428 \| HAVCR2 \| HAVcr-2 \| hepatitis A virus cellular receptor 2 \| kidney injury molecule-3 \| KIM-3 \| SPTCL \| T cell immunoglobulin mucin 3 \| T cell immunoglobulin mucin-3 \| T-cell immunoglobulin and mucin domain-containi
Specifications & Purity	Carrier Free, Azide Free, Validated, ≥95%(SDS-PAGE), See COA
Host species	Rat
Specificity	TIM 3/CD366
Immunogen	Recombinant protein of mouse TIM-3
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, Validated
Product Description	Rat anti Mouse TIM 3/CD366 Antibody, Monoclonal (RMT3-23), could be used for Flow, in vivo TIM-3 neutralization, in vitro TIM-3 blocking and so on. Application: Flow: Use at an assay dependent concentration. in vivo TIM-3 neutralization: Use at an assay dependent concentration. in vitro TIM-3 blocking: Use at an assay dependent concentration. Protein Function Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand (PubMed:24825777). Regulates macrophage activation (PubMed:11823861). Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance (PubMed:14556005). In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse (PubMed:24337741, PubMed:26492563). In contrast, shown to activate TCR-induced signaling in T-cells probably implicating ZAP70, LCP2, LCK and FYN (By similarity). Expressed on Treg cells can inhibit Th17 cell responses (PubMed:24838857). Receptor for LGALS9 (PubMed:16286920, PubMed:24337741). Binding to LGALS9 is believed to result in suppression of T-cell responses; the resulting apoptosis of antigen-specific cells may implicate HAVCR2 phosphorylation and disruption of its association with BAG6. Binding to LGALS9 is proposed to be involved in innate immune response to intracellular pathogens. Expressed on Th1 cells interacts with LGALS9 expressed on Mycobacterium tuberculosis-infected macrophages to stimulate antibactericidal activity including IL-1 beta secretion and to restrict intracellular bacterial growth (By similarity). However, the function as receptor for LGALS9 has been challenged (PubMed:23555261). Also reported to enhance CD8+ T-cell responses to an acute infection such as by Listeria monocytogenes (By similarity). Receptor for phosphatidylserine (PtSer); PtSer-binding is calcium-dependent. May recognize PtSer on apoptotic cells leading to their phagocytosis. Mediates the engulfment of apoptotic cells by dendritic cells. Expressed on T-cells, promotes conjugation but not engulfment of apoptotic cells. Expressed on dendritic cells (DCs) positively regulates innate immune response and in synergy with Toll-like receptors promotes secretion of TNF-alpha. In tumor-imfiltrating DCs suppresses nucleic acid-mediated innate immune repsonse by interaction with HMGB1 and interfering with nucleic acid-sensing and trafficking of nucleid acids to endosomes (By similarity). Expressed on natural killer (NK) cells acts as a coreceptor to enhance IFN-gamma production in response to LGALS9 (PubMed:22323453). In contrast, shown to suppress NK cell-mediated cytotoxicity (PubMed:22383801). Negatively regulates NK cell function in LPS-induced endotoxic shock.

Product Properties

Isotype	Rat IgG2a
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at 2-8°C,Do not freeze
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Associated Targets(Human)

HAVCR2 Tchem Hepatitis A virus cellular receptor 2 (0 Activities)

Discover Target: HAVCR2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Images

Flow Cytometry: TIM3/CD366 Rat mAb (Ab131268)

TIM3/CD366/PV-1 Rat mAb (Ab131268) - Flow Cytometry
Flow Cytometry analysis of RAW 264.7 cells labelling TIM3/CD366 (red) with TIM3/CD366 Rat mAb (Ab131268) at 0.1 μg/mL for 1 hour at 4°C. SA-PE (rp156150) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, Rat IgG (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
Flow Cytometry	Use at an assay dependent concentration.
in vivo TIM-3 neutralization	Use at an assay dependent concentration.
in vitro TIM-3 blocking	Use at an assay dependent concentration.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Alternative Products

Documents & Articles

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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TIM3/CD366 Rat mAb - Primary antibody, specific to TIM 3/CD366, Rat IgG2a

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

TIM3/CD366 Rat mAb - Primary antibody, specific to TIM 3/CD366, Rat IgG2a

Basic Description

Product Properties

Associated Targets(Human)

Mechanisms of Action

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews