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SPDP-C6-NHS ester - 90%, high purity , CAS No.158913-22-5

COA

Grade & Purity:

≥90%

Cas Number: 158913-22-5
Molecular Weight: 425.52
PubChem CID: 157808

In stock

Item Number

S595683

Grouped product items
SKU	Size	Availability	Price
S595683-25mg	25mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$113.90
S595683-100mg	100mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$361.90
S595683-250mg	250mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$722.90

Non-PEG linker

View related series

Non-PEG linker (187) PROTAC (2077) PROTAC Linkers (1898)

Basic Description

Synonyms	Propanamide, N-(6-((2,5-dioxo-1-pyrrolidinyl)oxy)-6-oxohexyl)-3-(2-pyridinyldithio)- \| Hexanoic acid, 6-[[1-oxo-3-(2-pyridinyldithio)propyl]amino]-, 2,5-dioxo-1-pyrrolidinyl ester \| MS-27499 \| 1-Phenyl-2,3-indolinedione, NSC 100013 \| MFCD00083163 \| N-(6-(
Specifications & Purity	≥90%
Storage Temp	Store at -20°C
Shipped In	Ice chest + Ice pads This product requires cold chain shipping. Ground and other economy services are not available.
Product Description	The amine-reactive portion of SPDP reagents is the N-hydroxysuccinimide (NHS) ester. Reactions are most commonly performed in phosphate, carbonate/bicarbonate, or borate buffers at pH 7-8. Other buffers can be used provided they do not contain primary amines (or thiols or disulfide reducing reagents – see discussion of the sulfhydryl reactivity). The rate of reaction and degradation by hydrolysis increases with increasing pH; for example, the half-life of the NHS ester is several hours at pH 7 and less than 10 minutes at pH 9. NHS-ester reagents like SPDP and LC-SPDP have limited aqueous solubility and must be dissolved in organic solvent before adding them to a reaction mixture. Sulfo-NHS-ester reagents like Sulfo-LC-SPDP are water-soluble and can be added directly to aqueous reaction mixtures. Features and advantages: Reactive groups: NHS ester and pyridyldithiol Reactive towards: amino and sulfhydryl groups Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5 Spacer arm is also easily cleaved using reducing SDS-PAGE sample loading buffer Cleavable crosslinker allows separation of crosslinked products Water-insoluble (dissolve first in DMF or DMSO) SPDP crosslinker is membrane permeable, so crosslinking can be done inside the cells Compare to other varieties of SPDP-type reagents, including pegylated forms

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
- Superclass Organoheterocyclic compounds
  - Class Pyridines and derivatives

Kingdom	Organic compounds
Superclass	Organoheterocyclic compounds
Class	Pyridines and derivatives
Subclass	Not available
Intermediate Tree Nodes	Not available
Direct Parent	Pyridines and derivatives
Alternative Parents	Pyrrolidine-2-ones Dicarboximides Heteroaromatic compounds Carboxylic acid salts Secondary carboxylic acid amides Lactams Organic disulfides Sulfenyl compounds Monocarboxylic acids and derivatives Azacyclic compounds Hydrocarbon derivatives Carbonyl compounds Organic oxides Organic salts Organonitrogen compounds Organopnictogen compounds
Molecular Framework	Aromatic heteromonocyclic compounds
Substituents	Pyridine - Pyrrolidone - 2-pyrrolidone - Dicarboximide - Pyrrolidine - Heteroaromatic compound - Carboxamide group - Carboxylic acid salt - Lactam - Organic disulfide - Secondary carboxylic acid amide - Carboxylic acid derivative - Monocarboxylic acid or derivatives - Sulfenyl compound - Azacycle - Organic oxide - Organopnictogen compound - Organic oxygen compound - Organosulfur compound - Organooxygen compound - Organonitrogen compound - Organic nitrogen compound - Hydrocarbon derivative - Carbonyl group - Organic salt - Aromatic heteromonocyclic compound
Description	This compound belongs to the class of organic compounds known as pyridines and derivatives. These are compounds containing a pyridine ring, which is a six-member aromatic heterocycle which consists of one nitrogen atom and five carbon atoms.
External Descriptors	Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61. DOI: 10.1186/s13321-016-0174-y

Names and Identifiers

IUPAC Name	(2,5-dioxopyrrolidin-1-yl) 6-[3-(pyridin-2-yldisulfanyl)propanoylamino]hexanoate
INCHI	InChI=1S/C18H23N3O5S2/c22-14(10-13-27-28-15-6-3-5-12-20-15)19-11-4-1-2-7-18(25)26-21-16(23)8-9-17(21)24/h3,5-6,12H,1-2,4,7-11,13H2,(H,19,22)
InChIKey	QYEAAMBIUQLHFQ-UHFFFAOYSA-N
Smiles	C1CC(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCSSC2=CC=CC=N2
Isomeric SMILES	C1CC(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCSSC2=CC=CC=N2
PubChem CID	157808
Molecular Weight	425.52

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number	Certificate Type	Date	Item
E2517065	Certificate of Analysis	Jul 08, 2024	S595683
G2415737	Certificate of Analysis	Jul 08, 2024	S595683
G2415738	Certificate of Analysis	Jul 08, 2024	S595683
G2415739	Certificate of Analysis	Jul 08, 2024	S595683

Chemical and Physical Properties

Sensitivity	Moisture Sensitive
Melt Point(°C)	92-94°C (lit.)
Molecular Weight	425.500 g/mol
XLogP3	1.000
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	13
Exact Mass	425.108 Da
Monoisotopic Mass	425.108 Da
Topological Polar Surface Area	156.000 Å²
Heavy Atom Count	28
Formal Charge	0
Complexity	545.000
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
The total count of all stereochemical bonds	0
Covalently-Bonded Unit Count	1

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