Determine the necessary mass, volume, or concentration for preparing a solution.
This is a demo store. No orders will be fulfilled.
| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab128237-10μl
|
10μl |
2
|
$66.90
|
|
|
Ab128237-50μl
|
50μl |
1
|
$189.90
|
|
|
Ab128237-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$299.90
|
|
|
Ab128237-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
|
|
mAb (4B10-B10-B6); Mouse anti Human SMAD5 Antibody; WB, IF, ICC, Flow; Unconjugated
| Product Name | SMAD5 Mouse mAb - Primary antibody, specific to SMAD5, Mouse IgG1 |
|---|---|
| Synonyms | Dwfc | hSmad5 | JV5-1 | JV5-1DKFZp781O1323 | MAD homolog 5 | MAD, mothers against decapentaplegic homolog 5 (Drosophila) | MADH5 | MADH5mothers against decapentaplegic homolog 5 | mothers against decapentaplegic homolog 5 | mothers against decapentaplegic |
| Specifications & Purity | ExactAb™, Validated, 1.6 mg/mL |
| Host species | Mouse |
| Specificity | SMAD5 |
| Immunogen | Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli. (AA 1-465). |
| Positive Control | WB: HeLa, SK-N-SH, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells. Flow: Jurkat cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description |
Mouse anti Human SMAD5 Antibody, Monoclonal (4B10-B10-B6), could be used for WB, IF/ICC, Flow and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | 1.6 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Recombinant SMAD5 Antibody (Ab128237) - Western Blot
All lanes: Recombinant SMAD5 Antibody (Ab128237) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 1.0 s
Recombinant SMAD5 Antibody (Ab128237) - IF/ICC
Immunocytochemistry analysis of Smad5 in HeLa cells using Recombinant SMAD5 Antibody (Ab128237).
Recombinant SMAD5 Antibody (Ab128237) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells stained with Recombinant SMAD5 Antibody (Ab128237) (red). Black line histogram represents the isotype control, normal mouse IgG.
| Application | Dilution info |
|---|---|
| WB | 1/500-1/1000 |
| IF/ICC | 1/50 - 1/200 |
| Flow Cytometry |
1/50-1/100
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Apr 22, 2024 | Ab128237 | |
| Certificate of Analysis | Apr 22, 2024 | Ab128237 |