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SMAD5 Mouse mAb - Primary antibody, specific to SMAD5, Mouse IgG1

    Application:
  • Flow Cytometry
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: 4B10-B10-B6
  • Conjugation: Unconjugated
In stock
Item Number
Ab128237
Grouped product items
SKU Size
Availability
Price Qty
Ab128237-10μl
10μl
2
$66.90
Ab128237-50μl
50μl
1
$189.90
Ab128237-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$299.90
Ab128237-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,699.90

mAb (4B10-B10-B6); Mouse anti Human SMAD5 Antibody; WB, IF, ICC, Flow; Unconjugated

Basic Description

Product Name SMAD5 Mouse mAb - Primary antibody, specific to SMAD5, Mouse IgG1
Synonyms Dwfc | hSmad5 | JV5-1 | JV5-1DKFZp781O1323 | MAD homolog 5 | MAD, mothers against decapentaplegic homolog 5 (Drosophila) | MADH5 | MADH5mothers against decapentaplegic homolog 5 | mothers against decapentaplegic homolog 5 | mothers against decapentaplegic
Specifications & Purity ExactAb™, Validated, 1.6 mg/mL
Host species Mouse
Specificity SMAD5
Immunogen Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli. (AA 1-465).
Positive Control WB: HeLa, SK-N-SH, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells. Flow: Jurkat cells.
Conjugation Unconjugated
Grade ExactAb™, Validated
Product Description

Mouse anti Human SMAD5 Antibody, Monoclonal (4B10-B10-B6), could be used for WB, IF/ICC, Flow and so on.
Application:
WB: 1/500-1/1000
IF/ICC: 1/50-1/200    
Flow: 1/50-1/100
Protein Function:
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 1.6 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

SMAD5 Tbio Mothers against decapentaplegic homolog 5 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant SMAD5 Antibody (Ab128237) - Western Blot
All lanes: Recombinant SMAD5 Antibody (Ab128237) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 1.0 s

Recombinant SMAD5 Antibody (Ab128237) - IF/ICC
Immunocytochemistry analysis of Smad5 in HeLa cells using Recombinant SMAD5 Antibody (Ab128237).

Recombinant SMAD5 Antibody (Ab128237) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells stained with Recombinant SMAD5 Antibody (Ab128237) (red). Black line histogram represents the isotype control, normal mouse IgG.

Application

Application Dilution info
WB

1/500-1/1000

IF/ICC

1/50 - 1/200

Flow Cytometry

1/50-1/100

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0404708 Certificate of Analysis Apr 22, 2024 Ab128237
ZJ24F0404707 Certificate of Analysis Apr 22, 2024 Ab128237

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