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sFlt-1 Mouse mAb - Primary antibody, specific to FLT1, Mouse IgG1

    Application:
  • Capture Antibody
  • CLIA
  • Detection Antibody
  • ELISA
  • FIA
  • FLFIA
  • GICA
  • LF
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: 1E4-6
  • Conjugation: Unconjugated
In stock
Item Number
Ab176421
Grouped product items
SKU Size
Availability
Price Qty
Ab176421-10μg
10μg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab176421-50μg
50μg
3
$199.90
Ab176421-100μg
100μg
3
$299.90
Ab176421-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb(1E4-6); Mouse anti Human sFlt-1 Antibody; Capture or Detection Antibody, ELISA, CLIA, LF, GICA, FIA; Unconjugated

Basic Description

Product Name sFlt-1 Mouse mAb - Primary antibody, specific to FLT1, Mouse IgG1
Synonyms EC 2.7.10.1 antibody | FLT 1 antibody | FLT antibody | Flt-1 antibody | FLT1 antibody | Fms like tyrosine kinase 1 antibody | Fms related tyrosine kinase 1 antibody | Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability
Specifications & Purity Carrier Free, ExactAb™, Azide Free, Validated, See COA
Host species Mouse
Specificity FLT1
Immunogen Recombinant Human sFlt-1 Protein, C-His tag
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human sFlt-1 Antibody, Monoclonal (1E4-6), is a Capture or Detection antibody could be used for ELISA, CLIA, LF, GICA, FIA.
CLIA: Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE
FLFIA: Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Affinity chromatography
Matched Pairs [Ab176419] sFlt-1 Mouse mAb as Detection or Capture antibody in ELISA, CLIA, LF, GICA, FIA
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 37°C short term(7 days)Store at 4°C short term(30 days). Store at -20±5°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

FLT1 Tclin Vascular endothelial growth factor receptor 1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)
FLT1 Tclin Vascular endothelial growth factor receptor 1 (6262 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID
FLT1 Tclin Vascular endothelial growth factor receptor (287 Activities)
Activity Type Relation Activity value Units Action Type Journal PubMed Id doi Assay Aladdin ID

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

Sandwich ELISA - sFlt-1 protein standard curve
Human sFlt-1 protein was serially diluted 2-fold and captured by sFlt-1 Mouse mAb (Ab176421) coated on a Clear Polystyrene Microplate (W291037). sFlt-1 Mouse mAb (Ab176419) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).


Relevance of detection results between aladdin Antibody Pair (Ab176421 & Ab176419) and Roche in CLIA
·Capture antibody: sFlt-1 Mouse mAb (Ab176421)
·Detection antibody: sFlt-1 Mouse mAb (Ab176419)

Relevance of detection results between aladdin Antibody Pair (Ab176419 & Ab176421) and Roche in CLIA
·Capture antibody: sFlt-1 Mouse mAb (Ab176419)
·Detection antibody: sFlt-1 Mouse mAb (Ab176421)

Binding ELISA - sFlt-1 Mouse mAb (Ab176421)
Immobilized Recombinant Human sFlt-1 Protein at 1.0 μg/mL can bind sFlt-1 Mouse mAb (Ab176421) with the EC₅₀ of 37.67 ng/mL.

Application

Application Dilution info
CLIA

Capture: 20µg/2mg magnetic beads; Detection: 100ug/4ug AE

FLFIA

Capture: Fluorescent beads/Antibody = 10/1; Detection: 1.0 mg/mL

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ23F1101566 Certificate of Analysis Nov 08, 2023 Ab176421
ZJ23F1101573 Certificate of Analysis Nov 08, 2023 Ab176421

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