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beta Tubulin Mouse mAb - Primary antibody, specific to beta tubulin, IgG3

COA COA
    Application:
  • Flow Cytometry
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG3
  • Clone Number: AB01/1E7
  • Conjugation: Unconjugated
In stock
Item Number
Ab179921
Grouped product items
SKU Size
Availability
 Price Qty
Ab179921-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$66.90
Ab179921-20μl
20μl
3
$99.90
Ab179921-50μl
50μl
4
$199.90
Ab179921-100μl
100μl
2
$299.90
Ab179921-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,499.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (AB01/1E7); Mouse anti Human beta tubulin Antibody; WB, Flow, ELISA; Unconjugated

View related series
Click to view target information: TUBB
Accession#:P07437 (8) beta Tubulin (7) Flow Cytometry (1158) Gene ID:203068 (8) TUBB (7)

Basic Description

Product Name beta Tubulin Mouse mAb - Primary antibody, specific to beta tubulin, IgG3
Synonyms Beta 4 tubulin antibody | Beta 5 tubulin antibody | beta Ib tubulin antibody | Beta1 tubulin antibody | Class I beta tubulin antibody | M40 antibody | MGC117247antibody | MGC16435 antibody | OK/SW cl.56 antibody | OK/SWcl.56 antibody | TBB5_HUMAN antibody
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High performance, 1.0mg/mL
Host species Mouse
Specificity beta tubulin
Immunogen Recombinant human beta tubulin
Positive Control WB: HeLa, A431, HEK293, MCF7, NIH3T3 and C6 whole cell lysates Flow: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Application
WB: 1/2,000-1/10,0000; Predicted molecular weight: 50 kDa
Flow: 0.002 μg/mL - 0.25 μg/mL
Protein Function:
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Product Properties

Isotype IgG3
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

TUBB Tclin Tubulin beta chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

beta Tubulin Mouse mAb (Ab179921) - Western Blot
All lanes: beta Tubulin Antibody (Ab179921) at 0.05 μg/mL
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 50.0 kDa
Observed band size: 50.0 kDa
Exposure time: 90.0 s 

beta Tubulin Mouse mAb (Ab179921) - Flow Cytometry
Overlay histogram showing HeLa cells stained with beta Tubulin Mouse mAb (Ab179921) (Red). Cells were fixed and permeabilized with 100% methanol. A Goat Anti-Mouse IgG H&L (FITC) (Ab156254) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG (Ab170221) was used as an isotype control (Blue).

Application

Application Dilution info
WB

1/2,000-1/10,0000; Predicted molecular weight: 50 kDa

Flow Cytometry

0.002 μg/mL - 0.25 μg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ23F1202057 Certificate of Analysis Dec 13, 2023 Ab179921
ZJ23F1202058 Certificate of Analysis Dec 13, 2023 Ab179921
ZJ23F1202059 Certificate of Analysis Dec 13, 2023 Ab179921

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