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Recombinant Human GM-CSF Protein, ＞98% (SDS-PAGE, HPLC ), high purity

Grade & Purity:

≥98%(SDS-PAGE&HPLC)

Expression System: E.coli
Accession #: P04141
Protein Tag: No tag
Bioactivity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is typically < 0.2ng/mL.
Endotoxin Concentration: <0.01 EU/μg

In stock

Item Number

rp146523

Grouped product items
SKU	Size	Availability	Price
rp146523-5μg	5μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$79.90
rp146523-10μg	10μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$129.90
rp146523-20μg	20μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$169.90
rp146523-100μg	100μg	Available within 4-8 weeks(?) Items will be manufactured post-order and can take 4-8 weeks. Thank you for your patience!	$489.90
rp146523-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,779.90

Animal Free, >98% (SDS-PAGE, HPLC ), Active, E.coli, No tag, 18-144aa

View related series

Click to view protein information: CSF2

Accession#:P04141 (10) CSF2 (14) Gene ID:1437 (10) GM-CSF (14)

Basic Description

Product Name	Recombinant Human GM-CSF Protein, ＞98% (SDS-PAGE, HPLC ), high purity
Synonyms	Human Granulocyte-Macrophage Colony Stimulating Factor \| Colony stimulating factor 2 (granulocyte-macrophage) \| Colony-stimulating factor \| CSF \| CSF2 \| CSF-2 \| GMCSF \| GM-CSF \| GMCSFgranulocyte-macrophage colony-stimulating factor \| granulocyte-macrophag
Grade	ActiBioPure™, Animal Free, Azide Free, Bioactive, Carrier Free, High Performance
Product Description	Purity ＞98% (SDS-PAGE; HPLC) . Purity is greater than 98% as determined by SEC-HPLC and reducing SDS-PAGE. Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Human Granulocyte-Macrophage Colony Stimulating Factor Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimulation. It was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors and has functions of stimulates the growth and differentiation of hematopoietic precursor cells from various lineages. GM-CSF has also been reported to have a functional role on non-hematopoietic cells and can induce human endothelial cells to migrate and proliferate. Additionally, it can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GM-CSF is used as a medication to stimulate the production of white blood cells following chemotherapy and has also recently been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The recombinant Human GM-CSF is a non-glycosylated polypeptide chain containing 127 amino acids.
Specifications & Purity	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥98%(SDS-PAGE&HPLC)
Purity	＞98% (SDS-PAGE, HPLC )
Bioactivity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is typically < 0.2ng/mL.
Endotoxin Concentration	<0.01 EU/μg
Expression System	E.coli
Species	Human
Amino Acids	18-144 aa
Sequence	APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFD LQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSC ATQIITFESFKENLKDFLLVIPFDCWEPVQE
Protein Tag	No tag
Protein Length	Full length protein
Accession #	P04141
Source	Recombinant
Predicted molecular weight	14.5 kD
SDS-PAGE	13.7 kDa, under reducing conditions; 12.3 kDa, under non-reducing conditions.

Storage and Shipping

Shape	Lyophilized
Reconstitution	1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom。 2、 Reconstitute in a sterile aqueous buffer to an appropriate concentration。 Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions。
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Upon receipt, it is recommended to aliquot. Store at -20°C, Avoid freeze / thaw cycles. Lyophilized with no additives. This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

Protein Bioactivity: Recombinant Human GM-CSF Protein (rp146523)

Recombinant Human GM-CSF Protein (rp146523)-Protein Bioactivity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is typically <0.2ng/mL.

SDS-PAGE: Recombinant Human GM-CSF Protein (rp146523)

Recombinant Human GM-CSF Protein (rp146523)-SDS-PAGE
3μg/lane of Recombinant Human GM-CSF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 13.7 kDa.

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4 results found

Lot Number	Certificate Type	Date	Item
ZJ23F0600490	Certificate of Analysis	Jul 03, 2023	rp146523
ZJ23F0600489	Certificate of Analysis	Jul 03, 2023	rp146523
ZJ23F0600488	Certificate of Analysis	Jul 03, 2023	rp146523
ZJ23F0600487	Certificate of Analysis	Jul 03, 2023	rp146523

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