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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
|
Ab214232-10T
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10T |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$39.90
|
|
|
Ab214232-25T
|
25T |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$79.90
|
|
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Ab214232-100T
|
100T |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$189.90
|
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Recombinant (P17L12); Mouse anti Human CD3ε Antibody (AF555); Flow Cytometry
| Product Name | Recombinant CD3 Antibody (AF555) - Primary antibody, specific to Human CD3ε |
|---|---|
| Synonyms | CD3 epsilon antibody | CD3e antibody | CD3e antigen antibody | CD3e antigen epsilon polypeptide (TiT3 complex) antibody | CD3E antigen epsilon polypeptide antibody | CD3E antigen, epsilon subunit antibody | CD3e molecule epsilon antibody | CD3e molecule, |
| Specifications & Purity | ExactAb™, Validated, 5 μL/test |
| Biochemical and Physiological Mechanisms | The CD3 complex mediates signal transduction. |
| Host species | Mouse |
| Specificity | Human CD3ε |
| Immunogen | Human Thymocytes |
| Positive Control | Flow Cytometry: Human peripheral blood lymphocytes |
| Conjugation | AF555 |
| Excitation(nm) | Green Laser(532nm) |
| Emission(nm) | 568nm |
| Ex/Em(nm) | Ex:553nm;Em:568nm |
| Grade | ExactAb™, Validated |
| Product Description |
Recombinant CD3ε Antibody, clone P17L12, could be used for Flow Cytometry. |
| Light Chain Type | kappa |
|---|---|
| Purification Method | Protein A purified |
| Concentration | 5 μL/test |
| Storage Temp | Store at 2-8°C,Protected from light,Do not freeze |
| Shipped In | Wet ice |
| Stability And Storage | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Recombinant CD3 Antibody (AF555) (Ab214232) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD3 Antibody (AF555) (Ab214232) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF555) (Ab190246).
Recombinant CD3 Antibody (AF555) (Ab214232) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD3 Antibody (AF555) (Ab214232) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF555) (Ab190246). Black - Unlabelled control, cells without incubation with primary antibody.
| Application | Dilution info |
|---|---|
| Flow Cytometry | 5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. |
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