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Recombinant CD3 Antibody (AF555) - Primary antibody, specific to Human CD3ε

    Application:
  • Flow Cytometry
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: P17L12
  • Conjugation: AF555
In stock
Item Number
Ab214232
Grouped product items
SKU Size
Availability
Price Qty
Ab214232-10T
10T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$39.90
Ab214232-25T
25T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab214232-100T
100T
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$189.90

Recombinant (P17L12); Mouse anti Human CD3ε Antibody (AF555); Flow Cytometry

Basic Description

Product Name Recombinant CD3 Antibody (AF555) - Primary antibody, specific to Human CD3ε
Synonyms CD3 epsilon antibody | CD3e antibody | CD3e antigen antibody | CD3e antigen epsilon polypeptide (TiT3 complex) antibody | CD3E antigen epsilon polypeptide antibody | CD3E antigen, epsilon subunit antibody | CD3e molecule epsilon antibody | CD3e molecule,
Specifications & Purity ExactAb™, Validated, 5 μL/test
Biochemical and Physiological Mechanisms The CD3 complex mediates signal transduction.
Host species Mouse
Specificity Human CD3ε
Immunogen Human Thymocytes
Positive Control Flow Cytometry: Human peripheral blood lymphocytes
Conjugation AF555
Excitation(nm) Green Laser(532nm)
Emission(nm) 568nm
Ex/Em(nm) Ex:553nm;Em:568nm
Grade ExactAb™, Validated
Product Description

Recombinant CD3ε Antibody, clone P17L12, could be used for Flow Cytometry.

Product Properties

Light Chain Type kappa
Purification Method Protein A purified
Concentration 5 μL/test
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Recombinant CD3 Antibody (AF555) (Ab214232) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood cells (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD3 Antibody (AF555) (Ab214232) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF555) (Ab190246).

Recombinant CD3 Antibody (AF555) (Ab214232) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD3 Antibody (AF555) (Ab214232) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF555) (Ab190246). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

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