Basic Description

Product Name	Recombinant Aprotinin - ≥3 EPU/mg, high purity , CAS No.9087-70-1
Synonyms	Aprotinin from bovine lung \| CCG-270718 \| APROTININ \| Traskolan \| Antilysin \| J-200086 \| Bovine Pancreatic Trypsin Inhibitor \| AKOS024457997
Grade	EnzymoPure™
Product Description	Aprotinin is a competitive serine protease inhibitor that inhibits trypsin,chymotrypsin,kallikrein and plasmin.Aprotinin forms stable complexes with and blocks the active sites of enzymes. Binding is reversible with most aprotinin,protease complexes and dissociating at pH >10 or <3. Effective concentration is equimolar with protease.Recombinant aprotinin is expressed in E. Coli, and purified with HPLC. It contains no animal-derived components. This is a recombinant form of bovine lung aprotinin, which is traditionally isolated from bovine lung by methods involving fractional precipitation, gel filtration, and ion exchange chromatography. UNIT DEFINITION:A conversion factor for Aprotinin is: 1 EPU = 1 USP Aprotinin Unit = 1800 KIU.
Specifications & Purity	EnzymoPure™, ≥3 EPU/mg
Biochemical and Physiological Mechanisms	Expressed in E. Coli

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
- Superclass Organic Polymers
  - Class Polypeptides

Kingdom	Organic compounds
Superclass	Organic Polymers
Class	Polypeptides
Subclass	Not available
Intermediate Tree Nodes	Not available
Direct Parent	Polypeptides
Alternative Parents	Cyclic peptides Phenylalanine and derivatives Pentacarboxylic acids and derivatives Aspartic acid and derivatives N-acyl-alpha amino acids Proline and derivatives Alpha amino acid amides Alanine and derivatives Amphetamines and derivatives Pyrrolidinecarboxamides N-acylpyrrolidines 1-hydroxy-2-unsubstituted benzenoids N-acyl amines Tertiary carboxylic acid amides Secondary alcohols Guanidines Amino acids Organic disulfides Secondary carboxylic acid amides Lactams Primary carboxylic acid amides Sulfenyl compounds Azacyclic compounds Carboximidamides Carboxylic acids Dialkylthioethers Primary alcohols Imines Carbonyl compounds Organopnictogen compounds Organic oxides Hydrocarbon derivatives Monoalkylamines
Molecular Framework	Aromatic heteropolycyclic compounds
Substituents	Polypeptide - Cyclic alpha peptide - Phenylalanine or derivatives - Pentacarboxylic acid or derivatives - Aspartic acid or derivatives - N-acyl-alpha amino acid or derivatives - N-acyl-alpha-amino acid - Proline or derivatives - Alpha-amino acid amide - Alanine or derivatives - Amphetamine or derivatives - N-substituted-alpha-amino acid - Alpha-amino acid or derivatives - N-acylpyrrolidine - Pyrrolidine carboxylic acid or derivatives - Pyrrolidine-2-carboxamide - 1-hydroxy-2-unsubstituted benzenoid - Phenol - Monocyclic benzene moiety - Fatty acyl - Benzenoid - N-acyl-amine - Fatty amide - Pyrrolidine - Tertiary carboxylic acid amide - Organic disulfide - Amino acid or derivatives - Primary carboxylic acid amide - Carboxamide group - Amino acid - Secondary alcohol - Secondary carboxylic acid amide - Lactam - Guanidine - Azacycle - Organoheterocyclic compound - Carboxylic acid derivative - Carboxylic acid - Dialkylthioether - Sulfenyl compound - Carboximidamide - Thioether - Hydrocarbon derivative - Organic oxide - Amine - Organopnictogen compound - Organic oxygen compound - Organic nitrogen compound - Carbonyl group - Alcohol - Imine - Primary aliphatic amine - Primary amine - Primary alcohol - Organosulfur compound - Organooxygen compound - Organonitrogen compound - Aromatic heteropolycyclic compound
Description	This compound belongs to the class of organic compounds known as polypeptides. These are peptides containing ten or more amino acid residues.
External Descriptors	Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61. DOI: 10.1186/s13321-016-0174-y

Associated Targets(Human)

PLG Tclin Plasminogen (0 Activities)

Discover Target: PLG

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Storage and Shipping

Concentration	≥3 EPU/mg
Storage Temp	Store at -20°C
Shipped In	Ice chest + Ice pads
CAS	9087-70-1
Unit definition	One trypsin inhibitor unit (EPU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-benzoyl-L-arginineethyl ether(BAEE) per sec at pH 7.6 at 25 °C.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

4 results found

Lot Number	Certificate Type	Date	Item
L2420689	Certificate of Analysis	Dec 13, 2024	R141091
I2312630	Certificate of Analysis	Sep 02, 2023	R141091
I2312629	Certificate of Analysis	Sep 02, 2023	R141091
L2203214	Certificate of Analysis	Oct 26, 2022	R141091

Genetic information

Alternate Names	Aprotinin from bovine lung \| CCG-270718 \| APROTININ \| Traskolan \| Antilysin \| J-200086 \| Bovine Pancreatic Trypsin Inhibitor \| AKOS024457997
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Cystathionine gamma-lyase overexpression inhibits cell proliferation via a H2S-dependent modulation of ERK1/2 phosphorylation and p21Cip/WAK-1., The Journal of biological chemistry, Yang, Guangdong G, Cao, Kun K, Wu, Lingyun L and Wang, Rui R. 7. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)., Genome research, Gerhard, Daniela S DS and 115 more authors. 8. Towards a proteome-scale map of the human protein-protein interaction network., Nature, Rual, Jean-François JF and 37 more authors. 9. The DNA sequence and biological annotation of human chromosome 1., Nature, Gregory, S G SG and 178 more authors. 10. Polymorphisms in one-carbon metabolism and trans-sulfuration pathway genes and susceptibility to bladder cancer., International journal of cancer, Moore, Lee E LE and 14 more authors. more 11. 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Integrative predictive model of coronary artery calcification in atherosclerosis., Circulation, McGeachie, Michael M and 13 more authors. 31. Cystathionine beta-synthase 844Ins68 polymorphism is not associated with the levels of homocysteine and cysteine in an Indian population., Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, Kumar, Jitender J, Garg, Gaurav G, Karthikeyan, Ganesan G and Sengupta, Shantanu S. 32. The expression and activity of cystathionine-γ-lyase and 3-mercaptopyruvate sulfurtransferase in human neoplastic cell lines., Amino acids, Jurkowska, Halina H, Placha, Wojciech W, Nagahara, Noriuki N and Wróbel, Maria M. 33. Ancient origin of the CTH alelle carrying the c.200C>T (p.T67I) variant in patients with cystathioninuria., Clinical genetics, Espinós, C C and 8 more authors. 34. Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study., Diabetes care, Bailey, Swneke D SD and 11 more authors. 35. Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia., PloS one, Jugessur, Astanand A and 12 more authors. 36. [Expression of cystathionine-γ-lyase/hydrogen sulfide pathway in CVB3-induced myocarditis in mice]., Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics, Hua, Wang W and 6 more authors. 37. Folate-metabolizing gene variants and pregnancy outcome of IVF., Reproductive biomedicine online, Laanpere, M M and 5 more authors. 38. Specificity protein-1 as a critical regulator of human cystathionine gamma-lyase in smooth muscle cells., The Journal of biological chemistry, Yang, Guangdong G, Pei, Yanxi Y, Teng, Huajian H, Cao, Qiuhui Q and Wang, Rui R. 39. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium., Briefings in bioinformatics, Gaudet, Pascale P, Livstone, Michael S MS, Lewis, Suzanna E SE and Thomas, Paul D PD. 40. Expression of cystathionine β-synthase and cystathionine γ-lyase in human pregnant myometrium and their roles in the control of uterine contractility., PloS one, You, Xing-Ji XJ and 8 more authors. 41. MicroRNA-21 represses human cystathionine gamma-lyase expression by targeting at specificity protein-1 in smooth muscle cells., Journal of cellular physiology, Yang, Guangdong G, Pei, Yanxi Y, Cao, Qiuhui Q and Wang, Rui R. 42. H2S-Induced sulfhydration of the phosphatase PTP1B and its role in the endoplasmic reticulum stress response., Science signaling, Krishnan, Navasona N, Fu, Cexiong C, Pappin, Darryl J DJ and Tonks, Nicholas K NK. 43. Characterization of hydrogen sulfide and its synthases, cystathionine β-synthase and cystathionine γ-lyase, in human prostatic tissue and cells., Urology, Guo, Hui H and 5 more authors. 44. Sp1 is involved in regulation of cystathionine γ-lyase gene expression and biological function by PI3K/Akt pathway in human hepatocellular carcinoma cell lines., Cellular signalling, Yin, Peng P and 12 more authors. 45. H₂S protects against pressure overload-induced heart failure via upregulation of endothelial nitric oxide synthase., Circulation, Kondo, Kazuhisa K and 14 more authors. 46. 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Neurodegeneration in Huntington's disease involves loss of cystathionine γ-lyase., Cell cycle (Georgetown, Tex.), Paul, Bindu D BD and Snyder, Solomon H SH. 62. The importance of MTHFR, MTR, MTRR and CSE expression levels in Caucasian women with preeclampsia., European journal of obstetrics, gynecology, and reproductive biology, Seremak-Mrozikiewicz, Agnieszka A and 6 more authors. 63. The importance of rs1021737 and rs482843 polymorphisms of cystathionine gamma-lyase in the etiology of preeclampsia in the Caucasian population., Ginekologia polska, Mrozikiewicz, Przemysław M PM and 8 more authors. 64. Cystathionine γ-lyase, a H2S-generating enzyme, is a GPBAR1-regulated gene and contributes to vasodilation caused by secondary bile acids., American journal of physiology. Heart and circulatory physiology, Renga, Barbara B and 9 more authors. 65. Correlation between the cystathionine-γ-lyase (CES) and the severity of peptic ulcer disease., African health sciences, Chen, Xiong X, Wan, Ying-Chun YC, Guo, Tao T, Xu, Can-Xia CX and Wang, Fen F. 66. MPST but not CSE is the primary regulator of hydrogen sulfide production and function in the coronary artery., American journal of physiology. Heart and circulatory physiology, Kuo, Maggie M MM and 10 more authors. 67. Transcriptional Regulation of Cystathionine-γ-Lyase in Endothelial Cells by NADPH Oxidase 4-Dependent Signaling., The Journal of biological chemistry, Mistry, Rajesh K RK and 8 more authors. 68. LPS Up-Regulates Cystathionine γ -Lyase Gene Expression in Primary Human Macrophages via NF-κB/ERK Pathway., Inflammation & allergy drug targets, Badiei, Alireza A, Gieseg, Steven S, Davies, Sian S, Izani Othman, Mohd M and Bhatia, Madhav M. 69. 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Dichotomous effects of isomeric secondary amines containing an aromatic nitrile and nitro group on human aortic smooth muscle cells via inhibition of cystathionine-γ-lyase., Biochimie, Ji, Yajing Y and 6 more authors. 74. Distinctive expression pattern of cystathionine-β-synthase and cystathionine-γ-lyase identifies mesenchymal stromal cells transition to mineralizing osteoblasts., Journal of cellular physiology, Gambari, Laura L and 6 more authors. 75. Age-Dependent Allergic Asthma Development and Cystathionine Gamma-Lyase Deficiency., Antioxidants & redox signaling, Wang, Peipei P and 6 more authors. 76. Augmented H2S production via cystathionine-beta-synthase upregulation plays a role in pregnancy-associated uterine vasodilation., Biology of reproduction, Sheibani, Lili L and 7 more authors. 77. Total sulfane sulfur bioavailability reflects ethnic and gender disparities in cardiovascular disease., Redox biology, Rajpal, Saurabh S and 12 more authors. 78. 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Alternative Products

Documents & Articles

Chromatin preparation from tissues for chromatin immunoprecipitation (ChIP)

Categories: Protocols

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Electrophoresis ChIP Lysis buffer Aprotinin

Citations of This Product

How to Cite Aladdin Scientific Products?

1. Liu Jing, Bai Xinpeng, Fu Pengcheng. (2022) In silico and in vitro assessment of bioactive peptides from Arthrospira platensis phycobiliproteins for DPP-IV inhibitory activity, ACE inhibitory activity, and antioxidant activity. JOURNAL OF APPLIED PHYCOLOGY, 34 (3): (1497-1511).
2. Erdong Yuan, Shiying Nie, Chunli Qi, Bo Chang, Jiaoyan Ren. (2021) Effect of Scomberomorus niphonius peptide on the characteristics of resveratrol. Food & Function, 12 (22): (11449-11459).
3. Fanglin Shen, Dan Li, Jianmin Chen. (2022) Mechanistic toxicity assessment of fine particulate matter emitted from fuel combustion via pathway-based approaches in human cells. SCIENCE OF THE TOTAL ENVIRONMENT, 806 (150214).
4. Jiantao Ping, Wenli Wu, Lubin Qi, Jie Liu, Jinpeng Liu, Binglu Zhao, Quanbo Wang, Li Yu, Jin-Ming Lin, Qiongzheng Hu. (2021) Hydrogel-assisted paper-based lateral flow sensor for the detection of trypsin in human serum. BIOSENSORS & BIOELECTRONICS, 192 (113548).
5. Jia-Yu Shi, Hai-Yan Pan, Kun Liu, Min Pan, Guo-Jun Si. (2020) Expression of ectopic trypsin in atherosclerotic plaques and the effects of aprotinin on plaque stability. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 690 (108460).
6. Jing Jin, Qiao‑Yun Hu, Wen‑Wen Xu, Wen‑Jia Zhu, Bei Liu, Jing Liu, Wei Wang, Hui‑Fang Zhou. (2019) Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary. Experimental and Therapeutic Medicine, 17 (5): (3501-3508).
7. Yunhua Zhang, Jinyun Ji, Xiao Zhao, Ruoheng Jin, Jianhong Li, Hu Wan. (2017) Expression, purification and enzymatic properties of a β-N-acetylhexosaminidase from brown planthopper, Nilaparvata lugens. JOURNAL OF ASIA-PACIFIC ENTOMOLOGY, 20 (1340).
8. Dandan Zhou, Xiaoxiao Li, Wencun Liu, Mingjun Zhang, Ying Cheng, Zhousong Xu, Jian Gao, Yiyang Wang. (2025) A novel approach for engineering DHCM/GelMA microgels: application in hepatocellular carcinoma cell encapsulation and chemoresistance research. Frontiers in Bioengineering and Biotechnology, 13
9. Xiaoxiao Li, Weikang Zhao, Dandan Zhou, Pei Li, Chen Zhao, Qiang Zhou, Yiyang Wang. (2024) Construction of Integral Decellularized Cartilage Using a Novel Hydrostatic Pressure Bioreactor. Tissue Engineering Part C-Methods, 30 (3): (113-129).
10. Ruixia Chen, Xiao-Chen Liu, Xianqi Yao, Wei Wang, Junyi Xiang, Igor Tomasevic, Weizheng Sun. (2024) Effects of high-pressure and CaCl2 pretreatments on the salt taste-enhancing activity of hydrolysate derived from spent hen meat. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE,
11. Feng Wu, Yili Xu, Chenyang Ji, Xue Li, Jue Shen, Yejie Fei, Guanghan Yuan, Hui Qian. (2025) Real-time global coagulation assay via ordered porous layer interferometry using silica colloidal crystal film. ANALYTICA CHIMICA ACTA, 1350 (343876).

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Recombinant Aprotinin - ≥3 EPU/mg, high purity , CAS No.9087-70-1