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RCAS1 Mouse mAb - Primary antibody, specific to RCAS1, Mouse IgG1

COA COA
    Application:
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: K40051_4H7
  • Conjugation: Unconjugated
In stock
Item Number
Ab124859
Grouped product items
SKU Size
Availability
 Price Qty
Ab124859-10μl
10μl
2
$69.90
Ab124859-50μl
50μl
1
$199.90
Ab124859-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$319.90
Ab124859-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (K40051_4H7); Mouse anti Human RCAS1 Antibody; WB, IHC; Unconjugated

View related series
Click to view target information: EBAG9
Accession#: O00559 (2) EBAG9 (2) Gene ID: 9166 (2) RCAS1 (1)

Basic Description

Product Name RCAS1 Mouse mAb - Primary antibody, specific to RCAS1, Mouse IgG1
Synonyms PDAF antibody | RCAS 1 antibody | RCAS1 antibody | RCAS1_HUMAN antibody | Receptor binding cancer antigen expressed on SiSo cells antibody | Receptor-binding cancer antigen expressed on SiSo cells antibody | EBAG9 | EB9 | PDAF | BAG9 antibody | Cancer ass
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species Mouse
Specificity RCAS1
Immunogen Recombinant human EBAG9 (AA 41-210).
Positive Control WB: SK-OV-3, MCF7, A549, Jurkat, HEK293, A431, NIH/3T3 and C6 cell lysates. IHC: Human brain tissue.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human RCAS1 Antibody, Monoclonal (K40051_4H7), could be used for WB, IHC and so on.
Application:
WB:1/1000
IHC:1/100
Protein Function:
May participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -80°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

EBAG9 Tbio Receptor-binding cancer antigen expressed on SiSo cells (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

RCAS1 Mouse mAb (Ab124859) - Western Blot
All lanes: RCAS1 Mouse mAb (Ab124859) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 31 kDa
Exposure time: 36.5 s

RCAS1 Mouse mAb (Ab124859) - IHC
IHC analysis of human brain tissue by RCAS1 Mouse mAb (Ab124859). IHC was performed using sections of the formalin-fixed paraffin-embedded human brain tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with RCAS1 Mouse mAb (Ab124859) at 10 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Glial cells and neuronal cells are positively stained at the cytoplasm.

Application

Application Dilution info
WB

1/1000

IHC

1/100

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot Number Certificate Type Date Item
ZJ24F0505560 Certificate of Analysis May 14, 2024 Ab124859
ZJ24F0505561 Certificate of Analysis May 14, 2024 Ab124859

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