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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab123682-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$79.90
|
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Ab123682-50μl
|
50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$239.90
|
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Ab123682-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$349.90
|
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Ab123682-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,599.90
|
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mAb (K70027_8C11); Mouse anti Human PSMD1 Antibody; WB, IHC; Unconjugated
| Product Name | PSMD1 Mouse mAb - Primary antibody, specific to PSMD1, Mouse IgG1 |
|---|---|
| Synonyms | MGC133041 antibody | 26S proteasome non ATPase regulatory subunit 1 antibody | 26S proteasome non ATPase subunit 1 antibody | 26S proteasome non-ATPase regulatory subunit 1 antibody | 26S proteasome regulatory subunit rpn2 antibody | 26S proteasome regula |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | PSMD1 |
| Immunogen | Recombinant protein of human PSMD1 (AA 791-920). |
| Positive Control | WB: Hep G2, HeLa, Jurkat, MCF7, A431, SK-OV-3, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human PSMD1 Antibody, Monoclonal (K70027_8C11), could be used for WB, IHC and so on. Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
PSMD1 Mouse mAb (Ab123682) - Western Blot
All lanes: PSMD1 Mouse mAb (Ab123682) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 108 kDa
Exposure time: 45.7 s
PSMD1 Mouse mAb (Ab123682) - IHC
IHC-P analysis of testis tissue by PSMD1 Mouse mAb (Ab123682).IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with PSMD1 Mouse mAb (Ab123682) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in seminiferous ducts are positively stained at the nuclei and cytoplasm.
| Application | Dilution info |
|---|---|
| WB |
1/1000
|
| IHC | 1/200
|