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PSMD1 Mouse mAb - Primary antibody, specific to PSMD1, Mouse IgG1

    Application:
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: K70027_8C11
  • Conjugation: Unconjugated
In stock
Item Number
Ab123682
Grouped product items
SKU Size
Availability
Price Qty
Ab123682-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$79.90
Ab123682-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$239.90
Ab123682-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$349.90
Ab123682-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,599.90

mAb (K70027_8C11); Mouse anti Human PSMD1 Antibody; WB, IHC; Unconjugated

Basic Description

Product Name PSMD1 Mouse mAb - Primary antibody, specific to PSMD1, Mouse IgG1
Synonyms MGC133041 antibody | 26S proteasome non ATPase regulatory subunit 1 antibody | 26S proteasome non ATPase subunit 1 antibody | 26S proteasome non-ATPase regulatory subunit 1 antibody | 26S proteasome regulatory subunit rpn2 antibody | 26S proteasome regula
Specifications & Purity ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Host species Mouse
Specificity PSMD1
Immunogen Recombinant protein of human PSMD1 (AA 791-920).
Positive Control WB: Hep G2, HeLa, Jurkat, MCF7, A431, SK-OV-3, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue.
Conjugation Unconjugated
Grade Carrier Free, ExactAb™, Validated
Product Description

Mouse anti Human PSMD1 Antibody, Monoclonal (K70027_8C11), could be used for WB, IHC and so on.
Application:
WB: 1/1000
IHC: 1/200 
Protein Function

Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.

Product Properties

Isotype Mouse IgG1
Light Chain Type kappa
SDS-PAGE 150 kDa
Purification Method Protein A purified
Shape Liquid
Concentration 1.0 mg/mL
Storage Temp Store at -80°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

PSMD1 Tbio 26S proteasome non-ATPase regulatory subunit 1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

PSMD1 Mouse mAb (Ab123682) - Western Blot
All lanes: PSMD1 Mouse mAb (Ab123682) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 108 kDa
Exposure time: 45.7 s

PSMD1 Mouse mAb (Ab123682) - IHC
IHC-P analysis of testis tissue by PSMD1 Mouse mAb (Ab123682).IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with PSMD1 Mouse mAb (Ab123682) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in seminiferous ducts are positively stained at the nuclei and cytoplasm.

Application

Application Dilution info
WB

1/1000

IHC

1/200

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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