Mn~84000 Da by NMR (equivalent to Mw 150-300 kDa by viscosity )
Biochemical and Physiological Mechanisms
This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the c
Storage Temp
Protected from light,Store at -20°C,Argon charged
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Product Description
Poly-D-lysine hydrobromide is a positively charged synthetic polyamino acid having one HBr per lysine unit. It is a crystalline solid soluble in water. Poly-D-lysine hydrobromide is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This polycation is resistant to enzymatic degradation and has been used to culture a wide variety of cell types.
Poly-D-lysine hydrobromide with a molecular weight greater than 30,000 Da is recommended for cell culture applications. The poly-D-lysine hydrobromide (MW=52,000 Da) is easier to use because it is less viscous in solution, however the poly-D-lysine hydrobromide with an higher molecular weight (MW=84,000 Da) has more attachement sites per polymer chain available to the cells. Both poly-D-lysine hydrobromide and poly-L-lysine hydrobromide can be used as a coating substrate, however certain cells digest poly-L-lysine hydrobromide. In this case, poly-D-lysine, as the attachment factor, is necessary to prevent cell disruption by excessive uptake of L-lysine.
Our poly-D-lysine hydrobromide has been purified by dialysis, sterile-filtered on 0.2um, lyophilized, and stored under Ar.
This compound belongs to the class of organic compounds known as d-alpha-amino acids. These are alpha amino acids which have the D-configuration of the alpha-carbon atom.
External Descriptors
Not available
1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.
1 Citations
E2322105