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Peroxidase from horseradish, CAS No.9003-99-0

72 Citations COA COA
    Grade & Purity:
  • Type I, essentially salt-free, lyophilized powder,≥50 units/mg solid (using pyrogallol)
  • Accession #: P00432
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Item Number
P755498
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Click to view protein information: LPO
Bacterial (3013) Glucose Metabolism (1943)

Basic Description

Product Name Peroxidase from horseradish, CAS No.9003-99-0
Product Description

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Application:

Peroxidase from horseradish has been used:
  • in fecal starch analysis
  • to measure plasma glucose levels by Trinder method
  • to incubate feruloylated arabinoxylan fractions and also to evaluate oxidative coupling
  • in the detection of glycoproteins
Specifications & Purity Type I, essentially salt-free, lyophilized powder,≥50 units/mg solid (using pyrogallol)
Biochemical and Physiological Mechanisms HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies
Accession # P00432
CAS 9003-99-0
Enzyme Commission Number 1.11.1.7(BRENDA,IUBMB)
Molecule Type Small molecule

Product Properties

Nacres NA.54

Storage and Shipping

Concentration Type I, essentially salt-free, lyophilized powder,≥50 units/mg solid (using pyrogallol)
Storage Temp Store at 2-8°C
Shipped In Wet ice

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

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