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Peroxidase from horseradish, CAS No.9003-99-0

72 Citations COA COA
    Grade & Purity:
  • Type X, ammonium sulfate suspension
  • Accession #: P00432
In stock
Item Number
P755413
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SKU Size
Availability
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P755413-2KU
2KU
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$82.90
P755413-5KU
5KU
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$144.90
P755413-10KU
10KU
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$268.90
P755413-25KU
25KU
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$525.90
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View related series
Click to view protein information: LPO
Bacterial (3013) Glucose Metabolism (1943)

Basic Description

Product Name Peroxidase from horseradish, CAS No.9003-99-0
Product Description

Horseradish peroxidase is isolated from horseradish roots (Amoracia rusticana) and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme. Its molecular weight (~44 kDa) includes the polypeptide chain (33,890 Daltons), hemin plus Ca2+ (~700 Daltons), and carbohydrate (~9,400 Daltons). At least seven isozymes of HRP exist. The isoelectric point for horseradish peroxidase isozymes ranges from 3.0 - 9.0.

Application:

Horseradish peroxidase (HRP) is isolated from horseradish roots (Amoracia rusticana). It is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. Horseradish peroxidase is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. Product P6140 has been used to detect low density lipoprotein (LDL).
The enzyme from Sigma has been used while assessing the skin sensitization potential of pro-haptens. It has also been used to show that peroxidase (PO) activity and its heat stability correlate with the availability of free Ca2+ ions.
Specifications & Purity Type X, ammonium sulfate suspension
Biochemical and Physiological Mechanisms HRP readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors. The optimal pH is 6.0-6.5 and the enzyme is most stable in the pH range of 5.0-9.0. HRP can be conjugated to antibodies
Accession # P00432
CAS 9003-99-0
Enzyme Commission Number 1.11.1.7(BRENDA,IUBMB)
Molecule Type Small molecule

Product Properties

Nacres NA.54

Storage and Shipping

Concentration Type X, ammonium sulfate suspension
Storage Temp Store at 2-8°C
Shipped In Wet ice

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

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