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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab121265-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
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Ab121265-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
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Ab121265-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$319.90
|
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Ab121265-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
|
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mAb (G51S157); Mouse anti Human Perilipin 3/TIP47 Antibody; WB, IF/ICC; Unconjugated
| Product Name | Perilipin 3/TIP47 Mouse mAb - Primary antibody, specific to PLIN3, Mouse IgG2a |
|---|---|
| Synonyms | Cargo selection protein TIP47 | M6PRBP1 | M6PRBP1MGC2012 | Mannose-6-phosphate receptor-binding protein 1 | perilipin 3,47 kDa MPR-binding protein | Perilipin3 | Perilipin-3 | Placental protein 17,47 kDa mannose 6-phosphate receptor-binding protein | PLIN |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network. |
| Host species | Mouse |
| Specificity | PLIN3 |
| Immunogen | KLH-conjugated synthetic peptide of human TIP47. |
| Positive Control | WB: K562 and U-2 OS cell lysates. IF/ICC: U-2 OS cells |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human Perilipin 3/TIP47 Antibody, Monoclonal (G51S157), could be used for WB, IF/ICC and so on. |
| Isotype | Mouse IgG2a |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
Perilipin 3/TIP47 Mouse mAb (Ab121265) - Western Blot
All lanes: Perilipin 3/TIP47 Mouse mAb (Ab121265) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 126.3 s
Perilipin 3/TIP47 Mouse mAb (Ab121265) - IF/ICC
Immunofluorescent analysis of TIP47 staining in U-2 OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Perilipin 3/TIP47 Mouse mAb (Ab121265) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
| Application | Dilution info |
|---|---|
| ICC/IF |
1/10-1/50
|
| WB |
1/500-1/1000
|