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Basic Description
| Storage Temp | Protected from light,Store at -20°C,Desiccated |
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| Shipped In |
Ice chest + Ice pads This product requires cold chain shipping. Ground and other economy services are not available. |
| Product Description |
Product introduction： NHS-Phosphine is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules. Features of NHS-Phosphine: • Soluble—reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples • Compatible—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents • Specific—NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides) • Chemoselective—azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiency This NHS-ester compound reacts to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine group (PPh2) reacts with azide-labeled molecules by a mechanism known asStaudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, this NHS-Phosphine reagent facilitates a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways. • Reactive Moiety:NHS Ester,Phosphine • Crosslinker Type:Heterobifunctional • Form:Powder: • Spacer:Short(<10 Å) • Labeling Method:Chemical Labeling • Spacer Arm Length:5.4 Å |
Product Properties
| Reactivity | Amine-Azide |
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Names and Identifiers
| Molecular Weight | 461.4 |
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Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Chemical and Physical Properties
| Solubility | DMF,DMSO |
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Solution Calculators
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