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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab213557-100μg
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100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$19.90
|
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Ab213557-1mg
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1mg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
|
|
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Ab213557-5mg
|
5mg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$279.90
|
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mAb (EF12/6D11); Mouse IgG1 Isotype Control Antibody; Flow Cytometry, ELISA; Unconjugated
| Product Name | Mouse IgG1-kappa, anti-E8L - Primary antibody, specific to E8L |
|---|---|
| Synonyms | Mouse IgG1, κ Isotype Control | Purified Mouse IgG1, kappa Isotype Control | Mouse IgG1 Isotype Control Antibody | Mouse IgG1 Isotype Control mAb | Immunoglobulin G1 Fc | Mouse IgG1 Isotype Control | Isotype control mAb for mouse IgG1 | IgG1 (Mouse) Isoty |
| Specifications & Purity | ExactAb™, Validated, Animal Free, Carrier Free, Azide Free, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chai |
| Host species | Mouse |
| Specificity | E8L |
| Immunogen | Recombinant protein corresponding to MPXV E8L |
| Positive Control | Flow Cytometry: Peripheral blood mononuclear cells; Mouse splenocytes |
| Conjugation | Unconjugated |
| Grade | Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse IgG1, Isotype Control Antibody (EF12/6D11), could be used for Flow Cytometry analysis, ELISA and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
Mouse IgG1-kappa, anti-E8L (Ab213557) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood mononuclear cells labelling CD40 (Red) with CD40 Mouse mAb (Ab094768) conjugated with biotin at 2.0 μg/mL for 1 hour at 4°C, compared to concentration matched Mouse IgG1-kappa, anti-E8L (Ab213557) (Blue) conjugated with biotin. Detected with SA-PE (rp156150).
Mouse IgG1-kappa, anti-E8L (Ab213557) - ICC/IF
IF analysis of HeLa cells using beta Tubulin Mouse mAb (Ab179922) (Green) compared to concentration matched Mouse IgG1-kappa, anti-E8L (Ab213557). The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. A Goat Anti-Mouse IgG H&L (FITC) (Ab179003) was used at a dilution of 1/1000. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| Flow Cytometry | Use at the same concentration as the detection antibody. |
Starting at $69.90
Starting at $69.90
Starting at $19.90