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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab115591-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$69.90
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Ab115591-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
|
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Ab115591-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$299.90
|
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Ab115591-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,399.90
|
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mAb (K16281_8D5); Mouse anti Human MMAB Antibody; WB, IHC; Unconjugated
| Product Name | MMAB Mouse mAb - Primary antibody, specific to MMAB, Mouse IgG1 |
|---|---|
| Synonyms | aquocob(I)alamin vitamin B12s adenosyltransferase antibody | ATP:cob(I)alamin adenosyltransferaseantibody | ATP:corrinoid adenosyltransferase antibody | ATRantibody | c-diamide adenosyltransferase antibody | cblB antibody | Cob antibody | Cob(I)alamin ade |
| Specifications & Purity | Validated, Carrier Free, ExactAb™, 1.0 mg/mL |
| Host species | Mouse |
| Specificity | MMAB |
| Immunogen | Recombinant protein of human MMAB (AA 41-240). |
| Positive Control | WB: A549, Hep G2 and HEK293 cell lysates. IHC: Human liver tissue. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human MMAB Antibody, Monoclonal (K16281_8D5), could be used for WB, IHC and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| Light Chain Type | kappa |
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
MMAB Mouse mAb (Ab115591) - Western Blot
All lanes: MMAB Mouse mAb (Ab115591) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 26 kDa
Exposure time: 43.4 s
MMAB Mouse mAb (Ab115591) - IHC
IHC-P analysis of liver tissue by MMAB Mouse mAb (Ab115591).IHC-P was performed using sections of the formalin-fixed paraffin-embedded liver tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with MMAB Mouse mAb (Ab115591) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Hepatocytes are positively stained at the cytoplasm.
| Application | Dilution info |
|---|---|
| WB |
1/1000
|
| IHC | 1/200
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Aug 20, 2024 | Ab115591 |