Grouped product items

SKU

Size

Availability

Price

Qty

Ab115281-10μg

10μg

$19.90

Ab115281-50μg

50μg

$59.90

Ab115281-100μg

100μg

$79.90

Ab115281-1mg

1mg

Available within 8-12 weeks(?)

$429.90

Basic Description

Product Name	I-A/I-E Rat mAb - Primary antibody, specific to I-A/I-E, Rat IgG2b
Synonyms	IAb \| Ia2 \| Ia-2 \| Abeta \| H-2Ab \| H2-Ab \| Rmcs1 \| I-Abeta \| Eb \| Ia4 \| H2Eb \| Ia-4 \| H-2Eb \| E-alpha-f \| H-2 class II histocompatibility antigen, E-K alpha chain \| H-2 class II histocompatibility antigen, E-U alpha chain \| H-2E \| H2-Ea \| H-2Ea \| H2-Ea-ps
Specifications & Purity	Carrier Free, ExactAb™, Azide Free, Validated, ≥95%(SDS-PAGE), See COA
Host species	Rat
Specificity	I-A/I-E
Immunogen	Activated C57BL/6 mouse spleen cells
Conjugation	Unconjugated
Grade	Azide Free, Carrier Free, ExactAb™, Validated
Product Description	Rat anti Mouse I-A/I-E Antibody, Monoclonal (M5/114), could be used for in vivo MHC II blockade, Functional assays, ICC, IF, WB, IP, Flow and so on. Application: in vivo MHC II blockade: Use at an assay dependent concentration. Functional assays: Use at an assay dependent concentration. ICC/IF: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. IP: Use at an assay dependent concentration. Flow: Use at an assay dependent concentration. Protein Function Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

Product Properties

Isotype	Rat IgG2b
Light Chain Type	kappa
SDS-PAGE	150 kDa
Purification Method	Protein G purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at 2-8°C,Do not freeze
Shipped In	Wet ice
Stability And Storage	Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Images

Flow Cytometry: I-A/I-E Rat mAb (Ab115281)

I-A/I-E Rat mAb (Ab115281) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling I-A/I-E with I-A/I-E Rat mAb (Ab115281) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150).

Flow Cytometry: I-A/I-E Rat mAb (Ab115281)

I-A/I-E Rat mAb (Ab115281) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling I-A/I-E (red) with I-A/I-E Rat mAb (Ab115281) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
WB	Use at an assay dependent concentration.
Functional Assay	Use at an assay dependent concentration.
IP	Use at an assay dependent concentration.
IF/ICC	Use at an assay dependent concentration.
Flow Cytometry	Use at an assay dependent concentration.
in vivo MHC II blockade	Use at an assay dependent concentration

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number	Certificate Type	Date	Item
ZJ24F0707965	Certificate of Analysis	Jul 09, 2024	Ab115281
ZJ24F0707966	Certificate of Analysis	Jul 09, 2024	Ab115281
ZJ24F0707967	Certificate of Analysis	Jul 09, 2024	Ab115281

Alternative Products

Documents & Articles

General Immunoprecipitation Protocol

Categories: Protocols

Tags:

Immunoprecipitation Western blot Cell lysate Co-immunoprecipitation IP Protein interaction Protein A/G magnetic beads

RNA Immunoprecipitation (RIP) Protocol

Categories: Protocols

Tags:

IP Protein A/G magnetic beads RNA immunoprecipitation RIP RNA protein interaction Isotype control antibody Input Crosslinking

Immobilized IP Protocol

Categories: Protocols

Tags:

Immunoprecipitation WB Western blot IP Protein interaction Immobilization immunoprecipitation Immobilized antibody

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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I-A/I-E Rat mAb - Primary antibody, specific to I-A/I-E, Rat IgG2b

Basic Description

Product Properties

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews

I-A/I-E Rat mAb - Primary antibody, specific to I-A/I-E, Rat IgG2b

Basic Description

Product Properties

Images

Application

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Related Products

Alternative Products

Documents & Articles

Solution Calculators

Molarity Calculator

Dilution Calculator

Reconstitution Calculator

Reviews

Customer Reviews