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HspBP1 Mouse mAb - Primary antibody, specific to HspBP1

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HspBP1 Mouse mAb - Primary antibody, specific to HspBP1

Grade & Purity:

See COA

Application:

Flow Cytometry
IHC
WB

Host species: Mouse
Reactive species: Human,Monkey,Mouse,Rat
Isotype: Mouse IgG1
Clone Number: OTI1D5
Conjugation: Unconjugated

In stock

Item Number

Ab108976

Grouped product items
SKU	Size	Availability	Price	Qty
Ab108976-10μl	10μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$79.90
Ab108976-50μl	50μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$229.90
Ab108976-100μl	100μl	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$359.90
Ab108976-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,899.90

mAb (OTI1D5); Mouse anti Human HspBP1 Antibody; WB,IHC, Flow Cytometry; Unconjugated

View related series

Click to view target information: HSPBP1

Accession#: Q9NZL4 (1) Flow Cytometry (1158) Gene ID: 23640 (1) HspBP1 (1)

Basic Description

Product Name	HspBP1 Mouse mAb - Primary antibody, specific to HspBP1
Synonyms	1500019G21Rik \| FES1 \| Heat shock protein 70 binding protein \| Heat shock protein 70 interacting protein \| Heat shock protein binding protein 1 \| Heat shock protein-binding protein 1 \| Heat-shock 70-KD protein-binding protein 1 \| HPBP1_HUMAN \| Hsp 70 bind
Specifications & Purity	ExactAb™, Validated, See COA
Biochemical and Physiological Mechanisms	Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.
Host species	Mouse
Specificity	HspBP1
Immunogen	Recombinant full length protein of human HspBP1 produced in HEK293T.
Positive Control	WB: Hep G2, HeLa, A549, Jurkat and MCF7 cell lysates. IHC: Human carcinoma of lung, ovary, pancreas, endometrium, adenocarcinoma of endometrium, carcinoma of bladder, lymphoma tissues. Flow Cytometry: HEK293T cells transfected with pCMV6-ENTRY HspBP1.
Conjugation	Unconjugated
Grade	ExactAb™, Validated
Product Description	Mouse anti Human HspBP1 Antibody, Monoclonal (OTI1D5), could be used for WB, IHC, Flow Cytometry and so on.

Product Properties

SDS-PAGE	150 kDa
Purification Method	Protein G purified
Shape	Liquid
Concentration	See COA
Storage Temp	Store at -20°C,Avoid repeated freezing and thawing
Shipped In	Ice chest + Ice pads
Stability And Storage	Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

HSPBP1 Tbio Hsp70-binding protein 1 (0 Activities)

Discover Target: HSPBP1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Images

Western Blot: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - Western Blot
All lanes: HspBP1 Mouse mAb (Ab108976) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 38 kDa
Exposure time: 11.4 s

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Carcinoma of Human lung tissue using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human ovary tissue using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Human endometrium tissue within the normal limits using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human endometrium tissue using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Carcinoma of Human bladder tissue using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - IHC
Immunohistochemical staining of paraffinembedded Human lymphoma tissue using HspBP1 Mouse mAb (Ab108976). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

Flow Cytometry: HspBP1 Mouse mAb (Ab108976)

HspBP1 Mouse mAb (Ab108976) - Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by HspBP1 Mouse mAb (Ab108976), and then analyzed by flow cytometry.

Application

Application	Dilution info
WB	1/500-1/2000
Flow Cytometry	1/100
IHC	1/150

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Documents & Articles

Flow Cytometry Using Methanol Permeabilization

Categories: Protocols

Tags:

Flow Cytometry FlowCytometry methanolpermeabilization flowcytometryantibodies Cell Sorting Cell Typing Immunology Research Intracellular Antigen Detection

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