Skip to the beginning of the images gallery

Goat Anti-Rabbit IgG H&L (LumiDye™ Red 720) - Secondary antibody, specific to Rabbit IgG

COA

Grade & Purity:

Validated

ExactAb™

1.0 mg/mL

Application:

IF/ICC

Host species: Goat
Reactive species: Rabbit
Isotype: Goat IgG
Conjugation: 176

In stock

Item Number

Ab220499

Grouped product items
SKU	Size	Availability	Price
Ab220499-10μg	10μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$37.90
Ab220499-100μg	100μg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$122.90
Ab220499-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$436.90

pAb; Goat Anti-Rabbit IgG H&L Antibody (LumiDye™ Red 720); IF/ICC

View related series

Goat Anti-Rabbit IgG (10) LumiDye™ Red 720 (2)

Basic Description

Product Name	Goat Anti-Rabbit IgG H&L (LumiDye™ Red 720) - Secondary antibody, specific to Rabbit IgG
Specifications & Purity	ExactAb™, Validated, 1.0 mg/mL
Biochemical and Physiological Mechanisms	IgG is the most abundant class of antibodies found in blood serum and lymph. It is active against bacteria, fungi, viruses, and foreign particles. Immunoglobulin G antibodies trigger action of the complement system.
Host species	Goat
Specificity	Rabbit IgG
Immunogen	Rabbit IgG
Conjugation	176
Excitation(nm)	Red Laser(633nm)
Emission(nm)	719nm
Ex/Em(nm)	Ex:696nm;Em:719nm
Grade	ExactAb™, Validated
SDS-PAGE	150kDa
Purification Method	Antigen affinity purified
Concentration	1.0 mg/mL
Product Description	Goat Anti-Rabbit IgG H&L Antibody, Secondary antibody, could be used for IF/ICC.

Storage and Shipping

Storage Temp	Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Stability And Storage	Store at -20°C. Avoid freeze/thaw cycle. Protect from light.

Images

IF/ICC: Goat Anti-Rabbit IgG H&L (LumiDye™ Red 720) (Ab220499)

Goat Anti-Rabbit IgG H&L (LumiDye™ Red 720) (Ab220499) - IF/ICC
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (LumiDye™ Red 720) (Ab220499) at a dilution of 1/500 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Application

Application	Dilution info
IF/ICC	1/100-1/1000

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration