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Goat Anti-Rabbit IgG H&L (AF647) (Ready to use) - Secondary antibody, specific to Rabbit IgG

COA

Grade & Purity:

Validated

ExactAb™

See COA

Application:

IF/ICC

Host species: Goat
Reactive species: Rabbit
Isotype: Goat IgG
Conjugation: AF647

In stock

Item Number

Ab218696

Grouped product items
SKU	Size	Availability	Price	Qty
Ab218696-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$15.90
Ab218696-10×1ml	10×1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$79.90

pAb; Goat Anti-Rabbit IgG H&L (AF647) (Ready to use); IF/ICC

View related series

AF647 (119) Goat Anti-Rabbit IgG H&L Antibody (15) Ready to use (23)

Basic Description

Product Name	Goat Anti-Rabbit IgG H&L (AF647) (Ready to use) - Secondary antibody, specific to Rabbit IgG
Synonyms	Goat Anti-Rabbit IgG H&L Antibody \| Goat Anti Rabbit IgG H&L Antibody \| Anti-Rabbit IgG H&L Antibody \| Anti Rabbit IgG H&L Antibody \| Goat Anti-Rabbit IgG Antibody \| Goat Anti Rabbit IgG Antibody \| Anti- Rabbit IgG Antibody \| Anti Rabbit IgG Antibody
Specifications & Purity	ExactAb™, Validated, See COA
Host species	Goat
Specificity	Rabbit IgG
Immunogen	Rabbit IgG
Conjugation	AF647
Excitation(nm)	Red Laser(633nm)
Emission(nm)	668nm
Ex/Em(nm)	Ex:650nm;Em:668nm
Grade	ExactAb™, Validated
SDS-PAGE	150kDa
Purification Method	Antigen affinity purified
Concentration	See COA
Product Description	Fluorescent secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from rabbit. Ready-to-use secondary antibody concentrations have been optimized and tested by immunofluorescent staining with IF/ICC. No calculations and dilutions are required. Just add an assay dependent volume to stain your cells, and you're ready for the next step in your experiment.

Storage and Shipping

Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	This product can be stored at 2-8°C for 12 months. Do not freeze. Store in the dark.

Images

IF/ICC: Goat Anti-Rabbit IgG H&L (AF647) (Ready to use) (Ab218696)

Goat Anti-Rabbit IgG H&L (AF647) (Ready to use) (Ab218696) - IF/ICC
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution in blocking buffer overnight at 4°C. Then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ready to use) (Ab218696) at 2mL/well for 1 hour at room temperature in the dark (red). A 6-well cell culture plate was used for this experiment. PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Application

Application	Dilution info
IF/ICC	Use at an assay dependent volume.

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

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