Skip to the beginning of the images gallery

Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) - Secondary antibody, specific to Mouse IgG

COA

Grade & Purity:

Validated

ExactAb™

See COA

Application:

Flow Cytometry
IF/ICC

Host species: Goat
Reactive species: Mouse
Isotype: Goat IgG
Conjugation: TRITC

In stock

Item Number

Ab220003

Grouped product items
SKU	Size	Availability	Price	Qty
Ab220003-1ml	1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$15.90
Ab220003-10×1ml	10×1ml	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$79.90

pAb; Goat Anti-Mouse IgG H&L Antibody (TRITC); Flow Cytometry, IF/ICC

View related series

Goat Anti-Mouse IgG H&L Antibody (18) Ready to use (23) TRITC (13)

Basic Description

Product Name	Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) - Secondary antibody, specific to Mouse IgG
Specifications & Purity	ExactAb™, Validated, See COA
Host species	Goat
Specificity	Mouse IgG
Immunogen	Mouse IgG
Conjugation	TRITC
Excitation(nm)	Green Laser(532nm)
Emission(nm)	576nm
Ex/Em(nm)	Ex:557nm;Em:576nm
Grade	ExactAb™, Validated
Purification Method	antigen affinity chromatography
Concentration	See COA
Product Description	Fluorescent secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from mouse. Ready-to-use secondary antibody concentrations have been optimized and tested by immunofluorescent staining with Flow Cytometry and IF/ICC. No calculations and dilutions are required. Just add an assay dependent volume to stain your cells, and you're ready for the next step in your experiment.

Storage and Shipping

Storage Temp	Store at 2-8°C,Protected from light,Do not freeze
Shipped In	Wet ice
Stability And Storage	This product can be stored at 2-8°C for 12 months. Do not freeze. Store in the dark.

Images

IF/ICC : Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) (Ab220003)

Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) (Ab220003) - IF/ICC
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed with 4% Paraformaldehyde Fix Solution (P395744) for 15 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with 1 μg/mL Catumaxomab (anti-CD3&EpCAM) (Ab175489) in blocking buffer overnight at 4°C. Then incubated with Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) (Ab220003) at 1mL/well for 1 hour at room temperature in the dark (red). A 24-well cell culture plate was used for this experiment. PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) (Ab220003)

Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) (Ab220003) - Flow Cytometry
Flow Cytometry analysis of Jurkat cells labelling CD45 (red) with Recombinant CD45 Antibody (Ab210608). Goat Anti-Mouse IgG H&L (TRITC) (Ready to use) (Ab220003) at 100 μL/well was used as the secondary antibody. Blue - Isotype control, Mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application	Dilution info
IF/ICC	Use at an assay dependent volume.
Flow Cytometry	100μL/test

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Solution Calculators

Molarity Calculator

Determine the necessary mass, volume, or concentration for preparing a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions, always use the batch-specific molecular weight of the product found on the vial label and the Certificate of Analysis (available online).

Dilution Calculator

Determine the dilution needed to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator enables you to quickly determine the volume of a reagent needed to reconstitute your vial. Just enter the mass of the reagent and the target concentration, and the calculator will do the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration