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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab220548-10μg
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10μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$12.90
|
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Ab220548-100μg
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100μg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$46.90
|
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Ab220548-1mg
|
1mg |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$279.90
|
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pAb; Goat Anti-Human IgG H&L Antibody (LumiDye™ Red 665); IF/ICC, Flow Cytometry
| Product Name | Goat Anti-Human IgG (LumiDye™ Red 665) - Primary antibody, specific to Human IgG |
|---|---|
| Synonyms | Goat Anti-Human IgG H&L Antibody | Goat Anti Human IgG H&L Antibody | Anti-Human IgG H&L Antibody | Anti Human IgG H&L Antibody | Human IgG H&L Antibody | Goat Anti-Human IgG Antibody | Goat Anti Human IgG Antibody | Anti-Human IgG Antibody | Anti Human I |
| Specifications & Purity | ExactAb™, Validated, 1.0 mg/mL |
| Host species | Goat |
| Specificity | Human IgG |
| Conjugation | 废弃Cy5 ®. Ex: 650nm, Em: 667nm |
| Excitation(nm) | Red Laser(633nm) |
| Emission(nm) | 665nm |
| Ex/Em(nm) | Ex:647nm;Em:665nm |
| Grade | ExactAb™, Validated |
| Purification Method | antigen affinity chromatography |
| Concentration | 1.0 mg/mL |
| Product Description |
Purified goat polyclonal antibody, suitable for Flow Cytometry and IF/ICC. |
| Storage Temp | Protected from light,Store at -20°C,Avoid repeated freezing and thawing |
|---|---|
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C. Avoid freeze/thaw cycle. Protect from light. |
Goat Anti-Human IgG (LumiDye™ Red 665) (Ab220548) - IF
IF analysis of EpCAM (red) in HT-29 cells. The cells were fixed and permeabilized with 4% PFA for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG (LumiDye™ Red 665) (Ab220548) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| Flow Cytometry |
1:100-1:2000
|
| IF/ICC |
1/100-1/1000
|