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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab175831-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$10.90
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Ab175831-100μl
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100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$39.90
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Ab175831-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$169.90
|
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pAb; Goat Anti-Human IgG H&L Antibody (Alkaline Phosphatase); WB, ELISA
| Product Name | Goat Anti-Human IgG (Alkaline Phosphatase) - Secondary antibody |
|---|---|
| Specifications & Purity | ExactAb™, Validated, See COA |
| Host species | Goat |
| Immunogen | human IgG whole molecule |
| Conjugation | Alkaline Phosphatase |
| Grade | ExactAb™, Validated |
| Purification Method | antigen affinity chromatography |
| Concentration | See COA |
| Product Description |
Purified goat polyclonal antibody, conjugated with Alkaline Phosphatase, suitable for WB and ELISA. |
| Storage Temp | Protected from light,Store at -20°C,Avoid repeated freezing and thawing |
|---|---|
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at -20°C. Avoid freeze/thaw cycle. Protect from light. |
Goat Anti-Human IgG (Alkaline Phosphatase) (Ab175831) - ELISA
Goat Anti-Human IgG (Alkaline Phosphatase) (Ab175831) was tested by direct ELISA. The plate was coated with 1 µg/mL (100 µL/well) Human IgG (Ab170213). A serial dilution of Goat Anti-Human IgG (Alkaline Phosphatase) (Ab175831) was used as the secondary antibody. Detection of alkaline phosphatase (AP) was performed using pNPP (P494193) after 30 minutes incubated at room temperature.
.
Goat Anti-Human IgG (Alkaline Phosphatase) (Ab175831) - Western Blot
All lanes: Goat Anti-Human IgG (Alkaline Phosphatase) (Ab175831)
at 1/1000 dilution
Samples: Lysates at 2.0 µg per lane
Detection of alkaline phosphatase (AP) was performed using BCIP/NBT (B494228) after 1hour incubated at room temperature.
Predicted band size: 50 kDa
Observed band size: 27, 53 kDa
| Application | Dilution info |
|---|---|
| ELISA |
1/1000-1/10000
|
| WB |
1/1000 - 1/20000
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