Basic Description

Synonyms	FITC \| Fluorescein isothiocyanate \| 3',6'-Dihydroxy-5(or 6)-isothiocyanatospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one \| Isothiocyanatofluorescein (mixture of 5- and 6- isomers)
Specifications & Purity	≥90%, mixture of 5- and 6- isomers
Biochemical and Physiological Mechanisms	Green fluorescent, amine-reactive label. Efficiently labels antibodies and other purified proteins at primary amines (lysine side chains). Used in applications including fluorescence microscopy, flow cytometry western blotting and ELISA.
Storage Temp	Store at 2-8°C
Shipped In	Wet ice This product requires cold chain shipping. Ground and other economy services are not available.
Note	Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description	Fluorescein 5(6)-isothiocyanate (FITC) is an isothiocyanate-derivitized Fluorescein fluorescent dye compound used for immunofluorescent identification and immunofluorescent histology applications. Fluorescein 5(6)-isothiocyanate reacts with nucleophilic amine and thiol amino acid residues to covalently link the compound to the protein of investigation. Fluorescein isothiocyanates have been described for use in the immunofluorescent identification of microorganisms and for the immunohistochemical localization of proteins.

Names and Identifiers

WGK Germany	3
Molecular Weight	389.38
Beilstein	1407295
Reaxy-Rn	1407295

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

15 results found

Lot Number	Certificate Type	Date	Item
G1922072	Certificate of Analysis	May 08, 2023	F299199
C2405048	Certificate of Analysis	Mar 15, 2023	F299199
D2312898	Certificate of Analysis	Mar 15, 2023	F299199
D2312892	Certificate of Analysis	Mar 15, 2023	F299199
D2312835	Certificate of Analysis	Mar 15, 2023	F299199
D2312695	Certificate of Analysis	Mar 15, 2023	F299199
D2312836	Certificate of Analysis	Mar 15, 2023	F299199
D2312893	Certificate of Analysis	Mar 15, 2023	F299199
J2227141	Certificate of Analysis	Nov 01, 2022	F299199
F2528069	Certificate of Analysis	Nov 15, 2021	F299199
K2401073	Certificate of Analysis	Nov 15, 2021	F299199
I2426397	Certificate of Analysis	Nov 15, 2021	F299199
A2508127	Certificate of Analysis	Nov 15, 2021	F299199
A2305182	Certificate of Analysis	Nov 15, 2021	F299199
H2409088	Certificate of Analysis	Nov 15, 2021	F299199

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Chemical and Physical Properties

Solubility	Soluble in DMSO (5 mg/ml), 2-methoxyethanol(9 mg/ml), acetone(1 mg/ml) and ethanol (20 mg/ml). It is soluble in water at less than 0.1 mg/ml.
Sensitivity	heat & light sensitive

Documents & Articles

Fluorescent Microparticles and Nanobeads

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Fluorescent labeling Fluorescent Particles Nanobeads Melamine Polyacrylonitrile Medicine Biochemistry Colloid Chemistry Aerosol Chromatographic Separation Medium Immobilized Enzyme Carrier Tracer

Citations of This Product

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Solution Calculators

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Reconstitution Calculator

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SKU	Size	Availability	Price
F299199-50mg	50mg	5	$19.90
F299199-250mg	250mg	3	$72.90
F299199-1g	1g	2	$209.90
F299199-5g	5g	3	$699.90

Fluorescein5(6)-isothiocyanate - 90%,mixture of 5- and 6- isomers, high purity , CAS No.27072-45-3