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FLI1 Mouse mAb - Primary antibody, specific to FLI1, Mouse IgG1

COA COA
    Application:
  • IHC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: CHFLI1
  • Conjugation: Unconjugated
In stock
Item Number
Ab103739
Grouped product items
SKU Size
Availability
 Price Qty
Ab103739-10μl
10μl
1
$69.90
Ab103739-50μl
50μl
1
$199.90
Ab103739-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$309.90
Ab103739-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,299.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (CHFLI1); Mouse anti Human FLI1 Antibody; WB, IHC; Unconjugated

View related series
Click to view target information: FLI1
Accession#: Q01543 (1) EWSR2 (1) FLI1 (1) Gene lD: 2313 (1)

Basic Description

Product Name FLI1 Mouse mAb - Primary antibody, specific to FLI1, Mouse IgG1
Synonyms BDPLT21 | ERGB | EWSR2 | FLI1 | Friend leukemia integration 1 transcription factor | Friend leukemia virus integration 1 | Proto-oncogene Fli-1 | SIC-1 | FLI-1 | Transcription factor ERGB | ERGB transcription factor antibody | Ewing Sarcoma breakpoint reg
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
Host species Mouse
Specificity FLI1
Immunogen A synthetic peptide derived from human FLI1
Positive Control WB: Jurkat, THP-1 and Ramos cell lysates. IHC: Human hemangioendothelioma and pnet tissues.
Conjugation Unconjugated
Grade ExactAb™, Validated
Product Description

Mouse anti Human FLI1 Antibody, Monoclonal (CHFLI1), could be used for WB, IHC and so on.

Product Properties

Isotype Mouse IgG1
SDS-PAGE 150 kDa
Purification Method Immunogen affinity purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

FLI1 Tbio Friend leukemia integration 1 transcription factor (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

FLI1 Mouse mAb (Ab103739) - Western Blot
All lanes: FLI1 Mouse mAb (Ab103739) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 51 kDa
Observed band size: 48, 52 kDa
FLI1 Mouse mAb (Ab103739) recognizes different isoforms of FLI1.
Exposure time: 60.0 s

FLI1 Mouse mAb (Ab103739) - Western Blot
All lanes: FLI1 Mouse mAb (Ab103739) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 51 kDa
Observed band size: 48, 51 kDa
FLI1 Mouse mAb (Ab103739) recognizes different isoforms of FLI1.
Exposure time: 100.0 s

FLI1 Mouse mAb (Ab103739) - IHC
Immunohistochemical analysis of FLI1 staining in human hemangioendothelioma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with FLI1 Mouse mAb (Ab103739) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

FLI1 Mouse mAb (Ab103739) - IHC
Immunohistochemical analysis of FLI1 staining in human pnet formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with FLI1 Mouse mAb (Ab103739) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Application

Application Dilution info
WB

1/500-1/1000
IHC

1/100-1/300

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

1 results found

Lot Number Certificate Type Date Item
ZJ24F0910093 Certificate of Analysis Sep 04, 2024 Ab103739

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