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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab103281-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$79.90
|
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Ab103281-50μl
|
50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$229.90
|
|
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Ab103281-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$359.90
|
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Ab103281-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,699.90
|
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mAb (K94041_8C12); Mouse anti Human FGF12 Antibody; WB, IP; Unconjugated
| Product Name | FGF12 Mouse mAb - Primary antibody, specific to FGF12 |
|---|---|
| Synonyms | EIEE47 antibody | FGF-12 antibody | Fgf12 antibody | FGF12_HUMAN antibody | FGF12B antibody | FHF-1 antibody | FHF1 antibody | Fibroblast growth factor 12 antibody | Fibroblast growth factor 12B antibody | Fibroblast growth factor FGF 12b antibody | Fibro |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Probably involved in nervous system development and function. |
| Host species | Mouse |
| Specificity | FGF12 |
| Immunogen | Recombinant protein of human FGF12 (AA 71-240) |
| Positive Control | WB: Rat brain, Mouse brain lysates. IP: Rat brain lysates. |
| Conjugation | Unconjugated |
| Grade | Carrier Free, ExactAb™, Validated |
| Product Description |
Mouse anti Human FGF12 Antibody, Monoclonal (K94041_8C12), could be used for WB, IP and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein A purified |
| Shape | Liquid |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -80°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
FGF12 Mouse mAb (Ab103281) - Western Blot
All lanes: FGF12 Mouse mAb (Ab103281) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 27 kDa
Observed band size: 22 kDa
Exposure time: 157.3 s
FGF12 Mouse mAb (Ab103281) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of K94041_8C12 with rat brain lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. FGF12 Mouse mAb (Ab103281) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody.
Lane 1: Rat brain lysate
Lane 2: FGF12 immunoprecipitated from rat brain lysate by FGF12 Mouse mAb (Ab103281)
Lane 3: The isotype control antibody instead of FGF12 Mouse mAb (Ab103281) in Rat brain lysate.
Result: FGF12 Mouse mAb (Ab103281) can immunoprecipitate FGF12.
| Application | Dilution info |
|---|---|
| WB |
1/1000
|
| IP | 1/100 |