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ER-Tracker Green - 98%, high purity , CAS No.730931-46-1

COA

Grade & Purity:

≥98%

Cas Number: 730931-46-1
Molecular Weight: 783.09

In stock

Item Number

E651376

Grouped product items
SKU	Size	Availability	Price
E651376-1mg	1mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$115.90
E651376-5mg	5mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$288.90
E651376-25mg	25mg	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$954.90

View related series

Fluorescent Dye (372)

Basic Description

Specifications & Purity	≥98%
Biochemical and Physiological Mechanisms	ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide ( HY-15206 ), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and form
Storage Temp	Protected from light,Store at -20°C,Desiccated
Shipped In	Ice chest + Ice pads This product requires cold chain shipping. Ground and other economy services are not available.
Product Description	ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide , highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K + channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining cells after fixation. In Vitro 1. Preparation of ER-Tracker working solution 1.1 Preparation of the stock solution Dissolve 100 ug ER-Tracker in 128 μL DMSO to obtain 1 mM of stock solution. Note: It is recommended to store the stock solution at -20℃or -80℃away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of ER-Tracker working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain 100 nM-1 μM of working solution. Note: Please adjust the concentration of ER-Tracker working solution according to the actual situation. 2. Cell staining 2.1 Suspension cells (6-well plate) a. Centrifuge at 1000 g at 4℃for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.The cell density is 1×10 6 /mL. b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes. c. Centrifuge at 400 g at 4℃for 3-4 minutes and then discard the supernatant. d. Wash twice with PBS, 5 minutes each time. e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 5-30 minutes. d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry. Note: If detection by flow cytometry, cells need to be resuspended before staining. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Form:Solid

Names and Identifiers

Smiles	O=C(NS(=O)(C1=CC=C(CCNC(C2=C(OC)C(NC(CCC3=CC=C4C=C5C(C)=CC(C)=[N]5[B+3]([F-])([N-]43)[F-])=O)=CC(Cl)=C2)=O)C=C1)=O)NC6CCCCC6
Molecular Weight	783.09

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):

Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

5 results found

Lot Number	Certificate Type	Date	Item
E2527687	Certificate of Analysis	May 16, 2025	E651376
E2527692	Certificate of Analysis	May 16, 2025	E651376
E2527686	Certificate of Analysis	May 16, 2025	E651376
E2527710	Certificate of Analysis	May 16, 2025	E651376
E2527707	Certificate of Analysis	May 16, 2025	E651376

Chemical and Physical Properties

Solubility	DMSO : 50 mg/mL (63.85 mM; Need ultrasonic)
Sensitivity	Moisture sensitive

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