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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab101289-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$69.90
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Ab101289-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$199.90
|
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Ab101289-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$289.90
|
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Ab101289-1ml
|
1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$2,399.90
|
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mAb (2E4F5E7); Mouse anti Human EIF2S1 Antibody; WB, IF/ICC; Unconjugated
| Product Name | EIF2S1 Mouse mAb - Primary antibody, Mouse IgG1 |
|---|---|
| Synonyms | Eukaryotic translation initiation factor 2 subunit 1 | eIF-2-alpha | eIF-2A | EIF2A | CDA 02 | CDA02 | eIF 2 alpha | EIF 2 | EIF 2A | EIF-2alpha | EIF2 | EIF2alpha | Eukaryotic Translation Initiation Factor 2 alpha | IF2A_HUMAN antibody | Eukaryotic trans |
| Specifications & Purity | ExactAb™, Validated, 0.5 mg/mL |
| Biochemical and Physiological Mechanisms | Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit |
| Host species | Mouse |
| Immunogen | Purified recombinant human eIF2α protein fragments expressed in E.coli. |
| Positive Control | WB: K562, HeLa, MCF7,C2C12, Hep G2, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells. |
| Conjugation | Unconjugated |
| Grade | ExactAb™, Validated |
| Product Description |
Mouse anti Human EIF2S1 Antibody, Monoclonal (2E4F5E7), could be used for WB, IF/ICC and so on. |
| Isotype | Mouse IgG1 |
|---|---|
| SDS-PAGE | 150 kDa |
| Purification Method | Protein G purified |
| Shape | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
| Mechanism of Action | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | References |
|---|
EIF2S1 Mouse mAb (Ab101289) - Western Blot
All lanes: EIF2S1 Mouse mAb (Ab101289) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 10.0 s
EIF2S1 Mouse mAb (Ab101289) - IF/ICC
Immunofluorescent analysis of EIF2S1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with EIF2S1 Mouse mAb (Ab101289) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
| Application | Dilution info |
|---|---|
| WB |
1/500-1/1000
|
| IF/ICC | 1/50-1/100 |