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EIF2S1 Mouse mAb - Primary antibody, Mouse IgG1

    Application:
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human,Mouse,Rat
  • Isotype: Mouse IgG1
  • Clone Number: 2E4F5E7
  • Conjugation: Unconjugated
In stock
Item Number
Ab101289
Grouped product items
SKU Size
Availability
Price Qty
Ab101289-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$69.90
Ab101289-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$199.90
Ab101289-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$289.90
Ab101289-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$2,399.90

mAb (2E4F5E7); Mouse anti Human EIF2S1 Antibody; WB, IF/ICC; Unconjugated

Basic Description

Product Name EIF2S1 Mouse mAb - Primary antibody, Mouse IgG1
Synonyms Eukaryotic translation initiation factor 2 subunit 1 | eIF-2-alpha | eIF-2A | EIF2A | CDA 02 | CDA02 | eIF 2 alpha | EIF 2 | EIF 2A | EIF-2alpha | EIF2 | EIF2alpha | Eukaryotic Translation Initiation Factor 2 alpha | IF2A_HUMAN antibody | Eukaryotic trans
Specifications & Purity ExactAb™, Validated, 0.5 mg/mL
Biochemical and Physiological Mechanisms Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit
Host species Mouse
Immunogen Purified recombinant human eIF2α protein fragments expressed in E.coli.
Positive Control WB: K562, HeLa, MCF7,C2C12, Hep G2, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells.
Conjugation Unconjugated
Grade ExactAb™, Validated
Product Description

Mouse anti Human EIF2S1 Antibody, Monoclonal (2E4F5E7), could be used for WB, IF/ICC and so on.

Product Properties

Isotype Mouse IgG1
SDS-PAGE 150 kDa
Purification Method Protein G purified
Shape Liquid
Concentration 0.5 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

EIF2S1 Tchem Eukaryotic translation initiation factor 2 subunit 1 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

EIF2S1 Mouse mAb (Ab101289) - Western Blot
All lanes: EIF2S1 Mouse mAb (Ab101289) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 10.0 s

EIF2S1 Mouse mAb (Ab101289) - IF/ICC
Immunofluorescent analysis of EIF2S1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with EIF2S1 Mouse mAb (Ab101289) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Application

Application Dilution info
WB

1/500-1/1000

IF/ICC

1/50-1/100

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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