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Basic Description
| Product Name | Dual Color Non-denatured Gel Protein Sample Loading Buffer |
|---|---|
| Product Description |
Aladdin's Dual Color Non-denaturing Protein Sample Loading Buffer (5X, Odorless) is an improved protein loading buffer with red and blue dyes, which can be used to load protein samples onto a non-denaturing PAGE gel for protein resolution.This product is safe to use. Instead of odoriferous DTT or 2-Mercaptoethanol reducing agent, this product employs an odorless and more stable reducing agent with similar reducing capability.This product has the same performance as conventional non-denaturing protein loading buffers.This product can be used for lane tracing after membrane transfer. It contains a special red dye which can be transferred to a PVDF or NC membrane to indicate the position of the lane, facilitating operations such as film cutting. Please refer to Figure 1 for the electrophoresis and the transfer results of this product.Figure 1. Aladdin's Dual Color Non-denaturing Protein Sample Loading Buffer (5X, Odorless) in the UltraBio™ Plus PAGE Precast Gel (Tris-Gly, 12%, 10 wells) after electrophoresis and transfer, as well as in the PVDF membrane after transfer (Blot). Precautions： This product must be completely dissolved and mixed well before use.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use： 1. Thaw this product at room temperature or in a water bath no more than 37℃. Place at room temperature immediately once dissolved and store at -20℃ after use.2. Add 1μl of this product per 4 μl of protein sample, and mix well. The mixture can be directly loaded to a gel.Note: After dilution to 1X, this product can also be used for sample lysis directly.3. Stop electrophoresis when the red or blue dye reaches the bottom of the gel. |
| Specifications & Purity | 5X, Odorless |
Storage and Shipping
| Concentration | 5X, Odorless |
|---|---|
| Storage Temp | Store at -20°C |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Stored at -20 ℃, valid for at least one year. |
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
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