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Inhibition of 5'-[gamma-32P]ATP-labelled 11-nucleotide ssDNA binding to recombinant Escherichia coli K-12 N-terminal His-tagged RNase T expressed in E...

Basic Information

ID: ALA3855161

Type: Binding

Description: Inhibition of 5'-[gamma-32P]ATP-labelled 11-nucleotide ssDNA binding to recombinant Escherichia coli K-12 N-terminal His-tagged RNase T expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL at 1 mM preincubated for 10 mins followed by substrate addition measured after 20 mins by TBE gel electrophoresis mobility shift assay

Format: BAO_0000019

Organism: Escherichia coli K-12

Target:  Ribonuclease T(ALA3879866)

Document:  ALA3853325

Inhibition of 5'-[gamma-32P]ATP-labelled 11-nucleotide ssDNA binding to recombinant Escherichia coli K-12 N-terminal His-tagged RNase T expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL at 1 mM preincubated for 10 mins followed by substrate addition measured after 20 mins by TBE gel electrophoresis mobility shift assay: 1

Activity Charts

Compound Summary

Parent Molecular Weight ALogP Polar Surface Area
422.35 2.45 2.45
192.12 -1.25 -1.25
495.28 5.20 5.20
396.36 3.70 3.70
393.48 4.95 4.95
206.16 1.77 1.77
393.21
193.27 -0.64 -0.64
518.44 4.31 4.31

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