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DAF-2 - ≥95% (HPLC), high purity , CAS No.205391-01-1

    Grade & Purity:
  • ≥95%(HPLC)
In stock
Item Number
D131457
Grouped product items
SKU Size
Availability
Price Qty
D131457-1mg
1mg
5
$308.90
D131457-5mg
5mg
3
$1,102.90
D131457-25mg
25mg
1
$4,959.90

Highly sensitive, specific fluorescent nitric oxide probe.

Basic Description

Synonyms 4,5-Diaminofluorescein | 2-(3,6-Dihydroxy-9H-xanthen-9-yl)-4,5-diamino-benzoic acid | DTXSID60443252 | AS-78359 | 5,6-Diamino-3',6'-dihydroxy-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one | DAF-2-CAS 205391-01-1-Calbiochem | Spiro[isobenzofuran-1(3H),9'-[9H]
Specifications & Purity ≥95%(HPLC)
Biochemical and Physiological Mechanisms Highly sensitive, specific fluorescent detector for nitric oxide (NO) . Essentially nonfluorescent until it reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T).
Storage Temp Store at -20°C
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Note Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Product Description

DAF-2 is a sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. In the presence of oxygen, the relatively non-fluorescent DAF-2 reacts rapidly with NO to yield the highly fluorescent triazolofluorescein compound. DAF-2 can detect NO under neutral conditions (detection limit = 5 nM).
A sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

Taxonomic Classification

Taxonomy Tree

Kingdom Organic compounds
Superclass Organoheterocyclic compounds
Class Benzopyrans
Subclass 1-benzopyrans
Intermediate Tree Nodes Dibenzopyrans
Direct Parent Xanthenes
Alternative Parents Diarylethers  Phthalides  Benzofuranones  Isobenzofurans  1-hydroxy-2-unsubstituted benzenoids  Lactones  Carboxylic acid esters  Amino acids and derivatives  Oxacyclic compounds  Primary amines  Organic oxides  Hydrocarbon derivatives  
Molecular Framework Aromatic heteropolycyclic compounds
Substituents Xanthene - Diaryl ether - Benzofuranone - Phthalide - Isobenzofuranone - Isocoumaran - Isobenzofuran - Phenol - 1-hydroxy-2-unsubstituted benzenoid - Benzenoid - Carboxylic acid ester - Lactone - Amino acid or derivatives - Ether - Carboxylic acid derivative - Oxacycle - Hydrocarbon derivative - Organooxygen compound - Organonitrogen compound - Organic oxide - Organic nitrogen compound - Organic oxygen compound - Amine - Primary amine - Aromatic heteropolycyclic compound
Description This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
External Descriptors Not available

Names and Identifiers

Pubchem Sid 504765573
Pubchem Sid Url https://pubchem.ncbi.nlm.nih.gov/substance/504765573
IUPAC Name 5,6-diamino-3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
INCHI InChI=1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
InChIKey LTYUPYUWXRTNFQ-UHFFFAOYSA-N
Smiles C1=CC2=C(C=C1O)OC3=C(C24C5=CC(=C(C=C5C(=O)O4)N)N)C=CC(=C3)O
Isomeric SMILES C1=CC2=C(C=C1O)OC3=C(C24C5=CC(=C(C=C5C(=O)O4)N)N)C=CC(=C3)O
Molecular Weight 362.34
Reaxy-Rn 8014486
Reaxys-RN_link_address https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=8014486&ln=

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

8 results found

Lot Number Certificate Type Date Item
G2322306 Certificate of Analysis May 09, 2025 D131457
G2322309 Certificate of Analysis May 09, 2025 D131457
G2322310 Certificate of Analysis May 09, 2025 D131457
G2322301 Certificate of Analysis May 09, 2025 D131457
G2322307 Certificate of Analysis May 09, 2025 D131457
G2322303 Certificate of Analysis May 09, 2025 D131457
L1902164 Certificate of Analysis Apr 09, 2025 D131457
L2322063 Certificate of Analysis Jan 02, 2024 D131457

Chemical and Physical Properties

Molecular Weight 362.300 g/mol
XLogP3 2.100
Hydrogen Bond Donor Count 4
Hydrogen Bond Acceptor Count 7
Rotatable Bond Count 0
Exact Mass 362.09 Da
Monoisotopic Mass 362.09 Da
Topological Polar Surface Area 128.000 Ų
Heavy Atom Count 27
Formal Charge 0
Complexity 589.000
Isotope Atom Count 0
Defined Atom Stereocenter Count 0
Undefined Atom Stereocenter Count 0
Defined Bond Stereocenter Count 0
Undefined Bond Stereocenter Count 0
The total count of all stereochemical bonds 0
Covalently-Bonded Unit Count 1

Solution Calculators

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