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| SKU | Size | Availability |
Price | Qty |
|---|---|---|---|---|
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Ab183857-10μl
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10μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
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$89.90
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Ab183857-50μl
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50μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$269.90
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Ab183857-100μl
|
100μl |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$426.90
|
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Ab183857-1ml
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1ml |
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
|
$1,999.90
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mAb (AB01/2G7); Mouse anti Human Cytokeratin 5; WB, IF/ICC, ELISA; Unconjugated
| Product Name | Cytokeratin 5 Mouse mAb - Primary antibody, specific to Human Cytokeratin 5 |
|---|---|
| Synonyms | 58 kDa cytokeratin antibody | CK-5 antibody | CK5 antibody | Cytokeratin-5 antibody | Cytokeratin5 antibody | DDD antibody | DDD1 antibody | EBS2 antibody | epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody | K2C5_HUMAN an |
| Specifications & Purity | ExactAb™, Validated, Carrier Free, Azide Free, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL |
| Biochemical and Physiological Mechanisms | Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modula |
| Host species | Mouse |
| Specificity | Human Cytokeratin 5 |
| Immunogen | Recombinant protein corresponding to Human Cytokeratin 5 |
| Positive Control | WB: A431, HeLa, HepG2 and SK-OV-3 whole cell lysates IF/ICC: HeLa cells |
| Conjugation | Unconjugated |
| Grade | Azide Free, Carrier Free, ExactAb™, High Performance, Validated |
| Product Description |
Mouse anti Human Cytokeratin 5 Antibody, clone AB01/2G7 , could be used for WB, IF/ICC, ELISA and so on. |
| Light Chain Type | kappa |
|---|---|
| SDS-PAGE | 150.0 KDa |
| Purification Method | Protein A purified |
| Concentration | 1.0 mg/mL |
| Storage Temp | Store at -20°C,Avoid repeated freezing and thawing |
| Shipped In | Ice chest + Ice pads |
| Stability And Storage | Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. |
| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
|---|
Cytokeratin 5 Mouse mAb (Ab183857) - Western Blot
All lanes: Cytokeratin 5 Mouse Antibody (Ab183857) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 58,65 kDa
Exposure time: 44.1 s
Cytokeratin 5 Mouse mAb (Ab183857) - ICC/IF
IF analysis of Cytokeratin 5 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Cytokeratin 5 Mouse mAb (Ab183857) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
| Application | Dilution info |
|---|---|
| IF/ICC | 1/200 |
| WB |
1/1000-1/10000
|
Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Date | Item |
|---|---|---|---|
| Certificate of Analysis | Dec 29, 2024 | Ab183857 |
Starting at $66.90