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Cytokeratin 5 Mouse mAb - Primary antibody, specific to Human Cytokeratin 5

    Application:
  • ELISA
  • IF/ICC
  • WB
  • Host species: Mouse
  • Reactive species: Human
  • Isotype: Mouse IgG1
  • Clone Number: AB01/2G7
  • Conjugation: Unconjugated
In stock
Item Number
Ab183857
Grouped product items
SKU Size
Availability
Price Qty
Ab183857-10μl
10μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$89.90
Ab183857-50μl
50μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$269.90
Ab183857-100μl
100μl
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$426.90
Ab183857-1ml
1ml
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$1,999.90

mAb (AB01/2G7); Mouse anti Human Cytokeratin 5; WB, IF/ICC, ELISA; Unconjugated

Basic Description

Product Name Cytokeratin 5 Mouse mAb - Primary antibody, specific to Human Cytokeratin 5
Synonyms 58 kDa cytokeratin antibody | CK-5 antibody | CK5 antibody | Cytokeratin-5 antibody | Cytokeratin5 antibody | DDD antibody | DDD1 antibody | EBS2 antibody | epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody | K2C5_HUMAN an
Specifications & Purity ExactAb™, Validated, Carrier Free, Azide Free, High Performance, ≥95%(SDS-PAGE), 1.0 mg/mL
Biochemical and Physiological Mechanisms Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modula
Host species Mouse
Specificity Human Cytokeratin 5
Immunogen Recombinant protein corresponding to Human Cytokeratin 5
Positive Control WB: A431, HeLa, HepG2 and SK-OV-3 whole cell lysates IF/ICC: HeLa cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Product Description

Mouse anti Human  Cytokeratin 5 Antibody, clone AB01/2G7 , could be used for WB, IF/ICC, ELISA and so on.

Product Properties

Light Chain Type kappa
SDS-PAGE 150.0 KDa
Purification Method Protein A purified
Concentration 1.0 mg/mL
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Associated Targets(Human)

KRT5 Tbio Keratin, type II cytoskeletal 5 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Images

Cytokeratin 5 Mouse mAb (Ab183857) - Western Blot
All lanes: Cytokeratin 5 Mouse Antibody (Ab183857) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 62 kDa
Observed band size: 58,65 kDa
Exposure time: 44.1 s 

Cytokeratin 5 Mouse mAb (Ab183857) - ICC/IF
IF analysis of Cytokeratin 5 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Cytokeratin 5 Mouse mAb (Ab183857) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Application

Application Dilution info
IF/ICC

1/200

WB

1/1000-1/10000

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

1 results found

Lot Number Certificate Type Date Item
ZJ24R1201621 Certificate of Analysis Dec 29, 2024 Ab183857

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