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Collagenase AF-1 From Clostridium Histolyticum - ≥ 3.00 U/mg, high purity , CAS No.9001-12-1

17 Citations

COA

Grade & Purity:

EnzymoPure™

≥ 3.00 U/mg

In stock

Item Number

C278962

Grouped product items
SKU	Size	Availability	Price	Qty
C278962-150U	150U	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$282.90
C278962-2000U	2000U	Available within 8-12 weeks(?) Production requires sourcing of materials. We appreciate your patience and understanding.	$2,747.90

Tissue dissociators, dissociated tissue and monolayer cells

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Basic Description

Product Name	Collagenase AF-1 From Clostridium Histolyticum - ≥ 3.00 U/mg, high purity , CAS No.9001-12-1
Synonyms	Collagenase I \| Collagenase Type II \| (5S,6S,9R,10S,13S,17S,23S,24S,27R,28S,31S,35S)-5,6,9,13,17,23,24,27,31,35-Decamethyl-10,28-dioctyl-2,20-diazanonacyclo[19.15.0.03,19.05,17.06,14.09,13.023,35.024,32.027,31]hexatriaconta-1(21),2,19-triene
Grade	EnzymoPure™
Product Description	Collagenase AF-1 is derived from C. histolyticum using a production process completely free of any animal based components. Thus, any risk of transmission of potential animal-derived pathogens is excluded. This highly purified collagenase, often in combination with Neutral Protease AF is suitable for the dissociation of sensitive cells from several tissues. The animal-free enzymes have been shown to be highly effective for the isolation of islets of Langerhans from human pancreas intended for transplantation and stromal vascular fraction (SVF) from adipose tissue and tumor cells. Application Collagenase AF-1 can be used for several cell isolations. Our bigger packaging size of 2000 U is ideal for human islet isolation, whereas the smaller size of 150 U can be used for tumor cell isolation, isolation of TILs or SVF from adipose tissue. Specifications Activity (Wünsch) ≥ 3.00 U/mg. Endotoxin ≤ 10.0 EU/mg (Ph. Eur.) Microbiological examination: Total aerobic microbial count (TAMC), total combined yeast/moulds count (TYMC), absence of Clostridia. Endproduct is 0.22 µm filtered. (Clostridiopeptidase A, Collagenase A) EC 3.4.24.3 • Mr Mr 70 000 - 120 000 (collagenase) • CAS [9001-12-1] References and Definitions Unit definitions:Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1. **Endotoxin: Ph. Eur. [1 Endotoxin Unit is equal to 1 International Unit of a WHO appoved reference standard endotoxin (RSE)]. References 1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-3091 2. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler´s Z. Physiol. Chem. 333, 149-51
Specifications & Purity	EnzymoPure™, ≥ 3.00 U/mg
CAS	9001-12-1
Enzyme Commission Number	3.4.24.3
Molecule Type	Small molecule

Associated Targets(Human)

HT-29 (80576 Activities)

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Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MCF7 (126967 Activities)

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SK-MEL-5 (47095 Activities)

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A549 (127892 Activities)

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Mechanisms of Action

Mechanism of Action	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	References

Storage and Shipping

Concentration	≥ 3.00 U/mg
Storage Temp	Store at -20°C
Shipped In	Ice chest + Ice pads
Unit definition	Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmol 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.

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Collagenase AF-1 From Clostridium Histolyticum - ≥ 3.00 U/mg, high purity , CAS No.9001-12-1

Basic Description

Associated Targets(Human)

Mechanisms of Action

Storage and Shipping

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

Citations of This Product

Solution Calculators

Molarity Calculator

Dilution Calculator

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