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CD47 Rat mAb (AF647) - Primary antibody, specific to CD47, Rat IgG2a

    Application:
  • Flow Cytometry
  • Host species: Rat
  • Reactive species: Mouse
  • Isotype: Rat IgG2a
  • Clone Number: MIAP301
  • Conjugation: AF647
In stock
Item Number
Ab190282
Grouped product items
SKU Size
Availability
Price Qty
Ab190282-10μg
10μg
4
$58.90
Ab190282-25μg
25μg
2
$117.90
Ab190282-100μg
100μg
1
$249.90

mAb (MIAP301); Rat anti Mouse CD47 Antibody (AF647); Flow

Basic Description

Product Name CD47 Rat mAb (AF647) - Primary antibody, specific to CD47, Rat IgG2a
Synonyms Itgp | 9130415E20Rik | B430305P08Rik | antigen identified by monoclonal 1D8 | Antigenic surface determinant protein OA3 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer) | CD47 antigen | CD47 glycoprotein | CD47 molecule | CD47 |
Specifications & Purity ExactAb™, Validated, Ex:650nm, Em:668nm, 0.5 mg/mL
Biochemical and Physiological Mechanisms Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, bind
Host species Rat
Specificity CD47
Immunogen Intact CD47 purified from placenta
Positive Control Flow: C57BL/6 mouse splenocytes.
Conjugation AF647
Excitation(nm) Red Laser(633nm)
Emission(nm) 668nm
Ex/Em(nm) Ex:650nm;Em:668nm
Grade Em:668nm, Ex:650nm, ExactAb™, Validated
Product Description

Rat anti Mouse CD47 Antibody, Monoclonal (MIAP301), could be used for Flow, in vivo CD47 blockade, in vitro CD47 blockade, IF, ICC and so on.


Product Properties

Isotype Rat IgG2a
Light Chain Type kappa
Purification Method Protein G purified
Concentration 0.5 mg/mL
Storage Temp Store at 2-8°C,Protected from light,Do not freeze
Shipped In Wet ice
Stability And Storage The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Associated Targets(Human)

CD47 Tchem Leukocyte surface antigen CD47 (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD47 Rat mAb (AF647) (Ab190282) - Flow Cytometry
Flow cytometry analysis of CD47 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD47 Rat mAb (AF647) (Ab190282) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application Dilution info
Flow Cytometry

≤1.0 μg/test, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume

Certificates(CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Solution Calculators

Reviews

Customer Reviews

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