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CD45R Rat mAb - Primary antibody, specific to CD45R, Rat IgG2a

COA COA
    Application:
  • ELISA
  • Flow Cytometry
  • Functional Assay
  • IF/ICC
  • IHC
  • IP
  • WB
  • Host species: Rat
  • Reactive species: Human,Mouse
  • Isotype: Rat IgG2a
  • Clone Number: RA3-6B2
  • Conjugation: Unconjugated
In stock
Item Number
Ab094928
Grouped product items
SKU Size
Availability
 Price Qty
Ab094928-10μg
10μg
10
$19.90
Ab094928-50μg
50μg
4
$29.90
Ab094928-100μg
100μg
2
$39.90
Ab094928-1mg
1mg
Available within 8-12 weeks(?)
Production requires sourcing of materials. We appreciate your patience and understanding.
$219.90
Wish List
Bulk Order  SDS  DATASHEET  Specification Sheet

mAb (RA3-6B2); Rat anti Mouse/Human CD45R Antibody; WB, Flow, IHC, IP, ICC, IF, ELISA, Functional Assays; Unconjugated

View related series
Click to view target information: PTPRC
Accession#:P06800 (28) Accession#:P08575 (56) CD45 (68) CD45R (34) Flow Cytometry (1158) Gene ID:19264 (28) Gene ID:5788 (52)

Basic Description

Product Name CD45R Rat mAb - Primary antibody, specific to CD45R, Rat IgG2a
Synonyms LCA | CD45 | T200 | GP180 | IMD105 | B220 | CD45 antigen | CD45R | EC 3.1.3.48 | L-CA | LY5 | protein tyrosine phosphatase, receptor type, C | receptor-type tyrosine-protein phosphatase C | T200 glycoprotein | T200 leukocyte common antigen | T200receptor
Specifications & Purity Carrier Free, Low Endotoxin, Azide Free, Validated, ≥95%(SDS-PAGE&HPLC), See COA
Host species Rat
Specificity CD45R
Immunogen Abelson murine leukemia virus-induced pre-B tumor cells
Conjugation Unconjugated
Grade Azide Free, Carrier Free, Low Endotoxin, Validated
Product Description

Rat anti Mouse/Human CD45R Antibody, Monoclonal (RA3-6B2), could be used for WB, Flow, IHC, IP, ICC, IF, ELISA,Functional Assays and so on.
Application
Flow: 0.125µg/100w cells  
IHC: 5.0µg/mL
WB: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
ELISA: Use at an assay dependent concentration.
Functional Assays: Use at an assay dependent concentration.
Protein Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

Product Properties

Isotype Rat IgG2a
Light Chain Type kappa
SDS-PAGE 26.4 kDa (Light Chain) & 50.4 kDa (Heavy Chain), under reducing conditions; 207.1 kDa, under non-reducing conditions.
Purification Method Protein A purified
Shape Liquid
Concentration See COA
Storage Temp Store at -20°C,Avoid repeated freezing and thawing
Shipped In Ice chest + Ice pads
Stability And Storage Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Associated Targets(Human)

PTPRC Tchem Receptor-type tyrosine-protein phosphatase C (0 Activities)
Activity Type Activity Value -log(M) Mechanism of Action Activity Reference Publications (PubMed IDs)

Mechanisms of Action

Mechanism of Action Action Type target ID Target Name Target Type Target Organism Binding Site Name References

Images

CD45R Rat mAb (Ab094928) - Flow Cytometry
Two-color flow cytometric analysis of CD45R expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD3 Rat mAb (FITC) (Ab185406) and either Rat IgG Isotype Control Antibody (Biotin) (Ab180310; Left panel) or CD45R Rat mAb (Ab094928; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD45R (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

CD45R Rat mAb (Ab094928) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD45R (red) with CD45R Rat mAb (Ab094928) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

CD45R Rat mAb (Ab094928) - Flow Cytometry
Flow Cytometry analysis of mouse splenocytes cells labelling CD45R with CD45R Rat mAb (Ab094928) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) (left panel) and detected with SA-PE (rp156150). 

Application

Application Dilution info
Flow Cytometry

0.125µg/100w cells
WB

Use at an assay dependent concentration.
Functional Assay

Use at an assay dependent concentration.
IP

Use at an assay dependent concentration.
IF/ICC

Use at an assay dependent concentration.
ELISA

Use at an assay dependent concentration.
IHC

5.0µg/mL

Certificates（CoA,COO,BSE/TSE and Analysis Chart)

C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot Number Certificate Type Date Item
ZJ24F0707947 Certificate of Analysis Jul 08, 2024 Ab094928
ZJ24F0707946 Certificate of Analysis Jul 08, 2024 Ab094928
ZJ24F0707945 Certificate of Analysis Jul 08, 2024 Ab094928

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